丹参骨髓腔内注射对兔股骨头坏死破骨细胞数量的影响

Influence of Intramedullary Given with Salvia miltiorrhiza Injection on Osteoclast of Steroid-induced Necrosis of Femoral Head in Rabbit

目的:复制激素性股骨头坏死动物模型,观察兔破骨细胞数量,从分子水平探讨丹参注射液骨髓腔内注射预防激素性股骨头缺血性坏死的可行性及机制。方法:选用家兔30只,随机分为对照组、模型组、丹参注射液预防组,每组10只。糖皮质激素诱导兔激素性股骨头坏死动物模型,造模同时预防组行骨髓腔内注射丹参注射液,每侧0.4 mg.kg-1,每周2次,连续8周。8周后观察3组家兔破骨细胞数量及组织病理学的改变。结果:预防组能有效的降低破骨细胞数量及股骨头空骨陷窝率。结论:丹参骨髓腔内注射可抑制破骨细胞生成,对早期激素性股骨头缺血性坏死有预防作用。

Objective：To copy the rabbit model of necrosis of the femoral head,observe the number of osteoclasts,study the feasibility and mechanism of intramedullary injection with Salvia miltiorrhiza injection for preventing of steroid-induced necrosis of the femoral head at the molecular level.Method： Thirty rabbits were randomly divided into three groups： control group,model group,prevention group,10 in each group.Glucocorticoid-induced rabbit model of femoral head necrosis was prepared.While making the models, the prevention group has received bone marrow cavity intramedullary injection with Salvia mihiorrhiza, at a dose of 0.4 mg·kg^-1 twice a week for 8 weeks. 8 weeks later, the number of osteoclasts and histopathological changes in three groups of rabbits were observed. Result： The prevention group could effectively reduce the number of osteoclasts and the rate of empty lacunae femoral head. Conclusion： Salvia mihiorrhiza injection plays a good effective role in the prevention of steroid-induced avascular necrosis of femoral head through inhibitting osteoclast formation.