赤芍总苷的制备及其对小鼠肝损伤的保护作用

Preparation of Total Glycosides of Paeonia lactiflora and Research on its Hepatoprotective Effects in Mice

目的:制备赤芍总苷,同时观察赤芍总苷对小鼠四氯化碳致急性肝损伤和DL-乙硫氨酸致急性脂肪肝的保护作用。方法:应用大孔树脂法,富集赤芍总苷部位。以四氯化碳致小鼠急性肝损伤模型和DL-乙硫氨酸致急性脂肪肝模型,考察赤芍总苷部位对小鼠血清丙氨酸氨基转移酶ALT及天冬氨酸氨基转移酶AST活性的影响,同时考察其对DL-乙硫氨酸致急性脂肪肝模型肝组织总胆固醇及甘油三酯含量的影响。结果:采用AB-8型大孔树脂,10%乙醇除杂,50%乙醇洗脱可得到含量50%以上的赤芍总苷部位。对四氯化碳致急性肝损伤模型,赤芍总苷可显著降低CCl4导致的小鼠血清ALT和AST活性的升高;对DL-乙硫氨酸致急性脂肪肝模型,各组小鼠血清ALT和AST活性无显著性差异,而模型组小鼠肝组织总胆固醇和甘油三酯含量显著升高,治疗后明显降低。结论:赤芍总苷对CCl4致小鼠急性肝损伤和DL-乙硫氨酸致小鼠急性脂肪肝具有一定的保护作用,其作用机制有待进一步实验研究。

Objective：To prepare the total glycosides of Paeonia lactiflora,and investigate its protection towards carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice.Method： Macroporous adsorption chromatography was used to enrich its total glycosides.Carbon tetrachloride induced acute liver injury and DL-ethionine induced acute fatty liver in mice were utilized to investigate the effect of total glycosides of P.lactiflora on serum alanine aminotransferase（ALT） and aspartate aminotransferase （AST） activities. Meanwhile, its effect on total cholesterol （TC） and triglyceride （TG） contents in liver was studied in DL-ethionine induced acute fatty liver model. Result： The polysaccharide and some other components was eluted off from the column of AB-8 resins with 10% ethanol, afterwards the remaining total glycosides was eluted off from the column by washing continuously with 50% ethanol, and its content was above 50%. On carbon tetrachloride induced acute liver injury model, the total glycosides could significantly reduce the increase of serum ALT and AST activities induced by CCl4; on DL-ethionine induced acute fatty liver model, there was no significant difference of serum ALT and AST activities between the model group and the control group, however, the content of total cholesterol and triglyceride in the liver of model groups significantly increased and decreased after treatment with total glycosides. Conclusion： The total glycosides of P. lactiflora has a protective effect on CCl4 induced acute liver injury and DL-ethionine induced acute fatty liver in mice.