山豆根致大鼠肝毒性研究

Hepatotoxicity of Rats Induced by Radix et Rhizoma Sophorae Tonkinensis

目的:观察山豆根长期大剂量给药后对大鼠产生的肝毒性反应。方法:SD大鼠,随机分为正常组、12 g.kg-1山豆根组、20 g.kg-1山豆根组,分别灌服蒸馏水、12 g.kg-1山豆根水煎液(0.6 g.mL-1)、20 g.kg-1山豆根水煎液(1 g.mL-1),连续给药26 d。观察给药期间的一般表现,体重变化等。末次给药后,用25℅乌拉坦腹腔麻醉,腹主动脉取血,离心,取血清,全自动生化仪检测ALT,AST,ALP,TbiL,TP,ALB,GLO等肝功能指标;摘取肝脏,称质量,计算脏器指数;按电镜要求,取肝脏组织,固定,透射电镜下观察超微结构。结果:大鼠连续灌胃山豆根水煎液10 d后,动物活动减少,四肢无力,粪便以及尿液增多;26d后,与正常组比较,ALT值及肝脏脏器指数升高(P<0.01或P<0.05),TP,ALB值降低(P<0.01或P<0.05);电镜显示,山豆根组动物肝脏细胞核变形、有肿胀趋势,核膜皱缩,毛细胆管扩张,滑面内质网略见扩张。结论:大鼠连续灌胃山豆根后,可对肝脏产生毒性,且随着剂量增加,毒性增强。

Objective：To detect the hepatic toxicity after giving large dose of Radix et Rhizoma Sophorae Tonkinensis（RRST） to rats for a long time.Method： The rats were divided into 3 groups（control group,12 g.kg-1 drug group,20 g.kg-1 drug group） and given the drug for 26 days.During the days,the rats’behave and weights were observed.After giving the drug for the last time,the rats were anaesthetized by 25% Urethane and the rats’blood was extracted from aorta abdominalis,then liver function was detected by using the blood serum. The rats＇ livers were taken and weighed, after that, the viscera index was calculated. According to the demand of electron microscope, hepatic tissues were fixed and ultrastructures were detected. Biochemical indicators such as ALT, AST, ALP, TBiL, TP, GLO and ALB were detected by using automatic analyzer. Result： After giving RRST for 10 days, the activity of the rats in drug group decreased, what＇ s more, the rats had general fatigue and the amount their excrement and urine increased. After giving drug for 26 days, compared with normal group, the ALT and viscera index increased in drug group （ P 〈 0.01 or P 〈 0.05 ） , the TP, ALB decreased in drug group （ P 〈 0.01 or P 〈0.05） ; Through the electron microscope, the results showed that in RRST group,the shape of nucleus changed, nucleus had the tendency of swelling, nuclear membranes shrunk, bile capillaries enlarged and smooth surfaced endoplasmic reticulum enlarged slightly. Conclusion： RRST can cause hepatic toxicity in rats by intragastric administration and the toxicity will increase if the dose of drug is added.