不同脂肪酸对灵芝液体发酵的影响

Effect of Fatty Acid Supplementation on Mycelium Biomass,Polysaccharide and Triterpene Production by Ganoderma lucidum Grown in Submerged Culture

研究硬脂酸、亚油酸和棕榈酸对灵芝液体发酵的影响。结果表明,3种供试脂肪酸都能促进灵芝菌丝体生物量的积累;硬脂酸有利于灵芝胞外多糖的产生,在添加浓度为1.0g/L时,胞外多糖量达到(1 066.74±11.27)mg/L,是对照的3.37倍;亚油酸利于胞内多糖的合成,在浓度为1.5g/L时,胞内多糖量达到(620.90±7.11)mg/L,是对照的2.56倍;另外,亚油酸还对灵芝胞内三萜和胞外三萜量表现出较强的促进作用,在浓度为2.0 g/L时,胞内及胞外三萜量分别达到(394.23±10.32)mg/L和(551.32±6.22)mg/L,分别比对照提高了1.13和1.12倍;而硬脂酸和棕榈酸的添加则降低胞内和胞外三萜的量。

The effects of supplementing Ganoderma lucidum growth medium with different concentrations of stearic,linoleic and palmitic acids were assessed in terms of mycelial biomass,extracellular and intracellular polysaccharide yields,and intracellular and extracellular triterpene levels.Mycelial biomass increased with increasing concentrations of all three fatty acids over the range 0-2.0 g/L.Highest yields of extracellular and intracellular polysaccharide were recorded using media supplemented with 1.0 g/L stearic acid and 1.5 g/L linoleic acid,respectively.Highest yields of extracellular and intracellular triterpenoids were recorded using medium supplemented with 2.0 g/L linoleic acid.