紫外线诱变建立草鱼抗出血病病原病毒的AHZC88细胞株

ESTABLISHMENT OF CELL STRAIN AHZC 88 RESISTANT TO PATHOGENIC VIRUSES OF GRASS CARP HEMORRHAGE BY ULTRAVIOLET (UV) INDUCEMENT

通过UV对草鱼出血病病原病毒敏感的草鱼ZC 7901细胞株反覆诱变,获得了对出血病病原病毒具有抗性的AHZC 88细胞株。经生物学特性测定,AHZC 88细胞的染色体2n=48,温度适应范围4°～38℃,最适生长温度27℃,分裂指数在36小时时达最高值。经病原病毒的人工侵染等试验表明,AHZC 88细胞不受草鱼出血病病毒感染。在电镜切片观察中,细胞内不见病毒颗粒。LDH同工酶分析,比敏感细胞少1条酶带。

The cell strain AHZC 88 of anti-hemorrhage of grass carp has been obtained by repeatedly UV inducement on strain ZC 7901 from the snout tissue cell sensitive to hemorrhage of grass carp. Identification of its biological characteristic showed that its chromosome number was 2n=48,the range of its suitable temperature was between 4-38℃, the optimal growth was 27℃, and mitotic index reached to maximum in 36 hours. The infective test with the pathogenic virus of hemorrhage proved that the cell strain AHZC 88 was not infected with the hemorrhage virus. The observation under electron microscope showed that there was no virosome in the cell of strain. The analysis of LDH isoenzyme displayed five zones which were different from the sensitive cell strain.