宫颈分泌物中survivin含量测定对宫颈癌诊断的初步评价

Primary evaluation of the value of detection of survivin concentration in cervical secretion for cervical cancer diagnosis

目的评估检测宫颈分泌物中凋亡抑制蛋白因子存活素(survivin)含量在宫颈癌诊断中的价值。方法用酶联免疫吸附法(ELISA)检测行宫颈活检(知情同意、自愿受试)患者的宫颈分泌物中survivin含量,采用受试者工作特征曲线(ROC)分析,通过计算ROC曲线下面积,确定切点值并分析评价检测指标的敏感性和特异性,并与液基薄层细胞学检查作比较。结果以survivin含量阳性的宫颈上皮内瘤变(CIN)2作为筛查标准,ROC曲线下面积=0.976,P<0.01,确定切点值为0.0595,Youden指数为0.828。敏感性85%,特异性97.8%。特异性明显优于液基薄层细胞学检查,但两者敏感性无显著性差异。结论检测宫颈分泌物中Survivin含量是一种好的宫颈癌辅助诊断方法。

Objective： To evaluate the value of detecting survivin concentration in cervical secretions for cervical cancer diagnosis. Methods： ELISA method was performed to detect the concentration of survivin in cervical secretions, cut-off value was determined by ROC curve. Sensitivity and specificity of surviving detection in cervical secretions for cervical cancer diagnosis were calculated and compared with liquid based cytology test. Results： Used cervical intra-epithelial neoplasia-2（CIN 2） as positive screening standard, the sensitivity and specificity of survivin positive detection was 85% and 97.8%. Compared with liquid based cytology test, the specificity of surviving detection was significantly higher（P〈0. 001）, while there was no statistical difference between their sensitivities. Conclusions： Detection of the survivin concentration in cervical secretions is a good assisted method for cervical cancer diagnosis.