绞股蓝皂甙对光损伤小鼠皮肤PCNA表达的影响

Effects of Ggypenosides(GP) on Expression of PCNA in Photo-damaged Mouse Skin

目的通过观察绞股蓝皂甙对光损伤小鼠皮肤中PCNA表达的影响,探讨绞股蓝皂甙抗光损伤的作用机理.方法中波紫外线(UVB)照射Balb/C小鼠建立光损伤模型,同时分别在照光前、后外擦1.5%绞股蓝皂甙霜对光损伤小鼠皮肤进行干预.运用免疫组织化学方法检测各组小鼠表皮中增殖细胞核抗原(proliferatingcell nuclearantigen,PCNA)的表达.具体分组:空白组、UVB模型组、GP组Ⅰ(后照光)、GP组Ⅱ(先照光)、VitE组Ⅰ(后照光)、VitE组Ⅱ(先照光)、基质组Ⅰ(后照光)、基质组Ⅱ(先照光).结果 (1)空白对照组Balb/C小鼠表皮中PCNA的表达低于UVB模型组(P>0.05);(2)GP组Ⅰ小鼠表皮中PCNA的表达低于UVB组(P<0.05);(3)GP组Ⅱ小鼠表皮中PCNA的表达低于UVB组;(4)VitE组Balb/C小鼠表皮PCNA的表达均与GP组表达相似.结论 1.5%GP抗光损伤的作用机制之一可能与通过下调小鼠表皮中PCNA表达量有关.

Objective To explore the mechanism of anti-photodamage effect of gypenosides（GP）by detecting the expression of proliferating cell nuclear antigen（PCNA）in photodamaged skin of Balb/C mice.Methods We established photo-damaged model of Balb/C mice with ultraviolet B（UVB）irradiation and used topical cream containing 1.5% GP to treat photo-damaged skin before and after UVB irradiation.The levels of PCNA was observed by immunohistochemistry（IHC）method.The mice in this study were randomly divided into eight groups：Blank group,UVB model group,Gypenosides group I（GP I）,Gypenosides group II（GP II）,Vitamine E group I（VitE I）,Vitamine E group II（VitE II）,Matrix group I,and Matrix group II.Results （1） The expression level of PCNA in Balb/C mouse skin in blank group was lower than that in UVB group（P0.05）.（2）The expression level of PCNA in Balb/C mouse skin in gypenosides groupⅠwas lower than that in UVB group（P0.05）.（3） The expression level of PCNA in Balb/C mouse skin in gypenosides group II was lower than that of UVB group.（4）The expression level of PCNA in VitE groups were similar to that in gypenosides groups.Conclusion One of the mechanisms of anti-photodamage of cream containing 1.5% GP is possibly related with downregualting the expression of PCNA in skin.