结直肠癌LoVo细胞株EMT逆转前后耐药相关ABC转运蛋白基因的变化

The change of drug-resistance related ABC gene expression in LoVo cell line of colorectal cancer before and after EMT

目的：观察结直肠癌LoVo细胞株发生上皮细胞间质转型（EMT）逆转前后3种耐药相关ABC转运蛋白基因的表达.方法：运用荧光定量PCR的方法,比较发生EMT的细胞株LoVo GnT-V/NC和EMT逆转之后的细胞株LoVo GnT-V/1564中 ABCB1、ABCC3、ABCB6基因表达量.结果：LoVo GnT-V/NC细胞中ABCB1基因表达量是LoVo GnT-V/1564的7.67倍;LoVo GnT-V/NC和LoVo GnT-V/1564细胞中ABCC3、ABCB6基因表达量差异均无统计学意义（P〉0.05）.结论：LoVo细胞株EMT发生后肿瘤细胞的耐药相关基因ABCB1的表达量也发生了改变,EMT可能与肿瘤的多药耐药相关.

Objective：To study the expression of ABC transport protein gene in colorectal cancer LoVo cell line before and after epithelial-mesenchymal transitions （EMT）. Methods： FQ-PCR （fluorescence quantitative polymerase chain reaction） was used to compare the expression of ABCBI ,ABCC3 and ABCB6 genes in LoVo GnT- V/NC cell line with EMT and in LoVo GnT-V/1564 cell line after EMT inversion. Results： The expression of ABCB1 in LoVo GnT-V/NC cells was 7.67 times to LoVo GnT-V/1564, while no statistical difference was found in the expression of ABCC3 and ABCB6 between LoVo GnT-V/NC and LoVo GnT-V/1564 （P 〉 O. 05）. Conclusions ： Expression of drug resistance related gene ABCB1 changed after EMT of LoVo cell line, indicating that EMT may be associated with multi-drug resistance （MDR） of tumor.