脾细胞输注致敏对异基因骨髓细胞损伤的机制

Mechanism of Impaired Allogeneic Bone Marrow Cells Caused by Sensitization of Spleen Cells Transfusion

【目的】探讨脾细胞输注致敏对异基因供者骨髓细胞损伤的作用机制。【方法】应用异基因脾细胞输注的BALB/c小鼠作为致敏模型,未致敏(正常)BALB/c小鼠作为对照,异基因供者骨髓细胞来源于C57BL/6小鼠。通过补体依赖细胞毒性反应(CDC)、细胞毒性免疫细胞杀伤作用、抗体依赖细胞介导的细胞毒性作用(ADCC)、粒-单核细胞集落培养(CFU-GM)等实验研究致敏血清及致敏免疫细胞对异基因骨髓细胞的影响。【结果】与补体孵育30min或1hr后,致敏血清组死亡细胞百分比明显高于正常血清组,其差异有统计学意义(P<0.001);细胞毒性免疫细胞杀伤作用及ADCC实验中致敏组的细胞毒性指数均高于正常组,其差异亦有统计学意义(P<0.05);致敏血清与骨髓细胞孵育组的CFU-GM数量比单纯骨髓细胞组有所减少,但两者差异无统计学意义(P>0.05)。【结论】致敏受者通过体液免疫及细胞免疫途径损伤异基因骨髓细胞。

【Objective】 To investigate the mechanism of impaired allogeneic donor bone marrow cells （BMCs） caused by sensitization of spleen cells transfusions. 【Methods】 BALB / c mice received allogeneic splenocytes transfusions were used as sensitized model,while non-sensitized BALB / c were used as normal controls. Allogeneic BMCs from C57BL / 6 were considered as donor cells. The effects of sensitized sera and immunity cells on donor BMCs were evaluated by the following tests：complement-dependent cytotoxicity （CDC）,antibody-dependent cellular cytotoxicity （ADCC）,Immune cell cytotoxicity and colony-forming units for granulocyte / monocyte （CFU-GM）. 【Results】 The percentages of dead cell in sensitized sera group were much higher than the control group after 30 min or 1 h incubation with complement,and the differences were statistically significant （P 0.001）. The cytotoxic indexes of immune cells and ADCC tests were higher in sensitized group comparing with control group,and the differences were statistically significant （P 0.05）. The numbers of CFU-GM in the group of BMCs with sensitized sera were lower than the group of BMCs alone,but no statistically significant differences were found between these two groups （P 0.05）. 【Conclusion】 Sensitization is cable of impairing allogeneic BMCs through humoral and cellular immunity pathways.