期刊文献+

肠易激综合征患者结肠黏膜辣椒素受体、P物质和肥大细胞变化的研究 被引量:16

Alterations of Capsaicin Receptor,Substance P and Mast Cells in Colonic Mucosa of Patients with Irritable Bowel Syndrome
下载PDF
导出
摘要 背景:腹痛是一般人群中最常见的肠道症状,肠易激综合征(IBS)患者的腹痛症状更为严重、频繁,然而其发生机制尚不明确。目的:分析IBS患者结肠黏膜中与痛觉和内脏高敏感相关的辣椒素受体VRI、P物质(SP)和肥大细胞(MC)的变化,探讨IBS患者内脏高敏感和腹痛的发生机制。方法:39例IBS患者[腹泻型IBS(IBS—D)21例,便秘型IBS(IBS-C)18例]和18名健康人纳入研究。受检者于结肠镜检查时在回盲部和乙状结肠取活检,行VR1、SP免疫组化染色和MC改良甲苯胺蓝染色。结肠镜检查前采用视觉模拟评分法行疼痛评分。结果:IBS患者乙状结肠VR1、回盲部和乙状结肠SP免疫反应阳性细胞以及回盲部MC数量显著多于正常对照组(P〈0.01),IBS-D与IBS-C组间则无明显差异。IBS—D和IBS—C患者的腹痛评分均与VR1呈正相关(r=0.553,P=O.009;r=0.592,P=0.010)。结论:IBS患者结肠黏膜中VR1、SP免疫反应阳性细胞和MC数量显著增多,VR1与腹痛评分呈正相关,三者可能参与了IBS患者内脏高敏感和腹痛的发生机制。 Background: Abdominal pain is the most commonly seen intestinal symptom in general population and being more severe and frequent in patients with irritable bowel syndrome (IBS), but the mechanisms underlying abdominal pain in IBS are poorly understood. Aims: To investigate the alterations of capsaicin receptor VR1, substance P (SP) and mast cells (MC), which are correlated with pain sensation and visceral hypersensitivity in colonic mucosa of IBS patients, and the pathogenesis of visceral hypersensitivity and abdominal pain in IBS. Methods: Ileocecal and sigmoid biopsies were obtained from 39 IBS patients, including 21 diarrhea-predominant IBS (IBS-D), 18 constipation-predominant IBS (IBS-C), and 18 healthy subjects during colonoscopy. VRI and SP were quantified following immunohistoehemical stain, and modified toluidine blue stain performed for assessing MC. The severity of abdominal pain was scored by visual analogue scale before colonoscopy. Results: A significant increase in numbers of VR1 and SP immunoreactive ceils in ileocecal and/or sigmoid mucosa, as well as in number of MC in ileocecal mucosa, was observed in biopsies from IBS patients as compared with the controls (P〈0.01), while no significant difference was found between IBS-D and IBS-C. In correlation analysis, the scores of abdominal pain in IBS-D and IBS-C were positively correlated with the number of VR1 immunoreactive cells (r=0.553, P=0.009; r=0.592, P=0.010). Conclusions: The numbers of VRI, SP immunoreactive cells and MC in colonic mucosa of IBS patients are increased significantly; furthermore, VR1 is positively correlated with the score of abdominal pain. These findings suggest that VRI, SP and MC might contribute to the visceral hypersensitivity and abdominal pain in patients with IBS.
出处 《胃肠病学》 2010年第11期672-675,共4页 Chinese Journal of Gastroenterology
基金 上海市嘉定区科技发展基金资助(2008JKK026)
关键词 肠易激综合征 腹痛 内脏高敏感 TRPV阳离子通道 P物质 肥大细胞 Irritable Bowel Syndrome Abdominal Pain Visceral Hypersensitivity TRPV Cation Channels Substance P Mast Ceils
  • 相关文献

参考文献17

  • 1Heaton KW, O'Donnell LJ, Braddon FE, et al. Symptoms of irritable bowel syndrome in a British urban community: consulters and nonconsulters. Gastroenterology, 1992, 102 (6): 1962-1967.
  • 2陈永萍,李任先,张万岱,陈村龙,梁卫江.肠易激综合征不同中医证型的神经内分泌学研究[J].第一军医大学学报,2001,21(3):202-204. 被引量:11
  • 3Davis JB, Gray J, Gunthorpe MJ, et al. Vanilloid receptor- 1 is essential for inflammatory thermal hyperalgesia. Nature, 2000, 405 (6783): 183-187.
  • 4Szallasi A, Blumberg PM. Vanilloid (Capsaicin) receptors and mechanisms. Pharmacol Rev, 1999, 51 (2): 159-212.
  • 5Geppetti P, Trevisani M. Activation and sensitisation of the vanilloid receptor: role in gastrointestinal inflammation and function. Br J Pharmacol, 2004, 141 (8): 1313-1320.
  • 6Winston J, Shenoy M, Medley D, et al. The vanilloid receptor initiates and maintains colonic hypersensitivity induced by neonatal colon irritation in rats. Gastroenterology, 2007, 132 (2): 615-627.
  • 7Miranda A, Nordstrom E, Mannem A, et al. The role of transient receptor potential vanilloid 1 in mechanical and chemical visceral hyperalgesia following experimental colitis. Neuroscience, 2007, 148 (4): 1021-1032.
  • 8Yiangou Y, Facer P, Dyer NH, et al. Vanilloid receptor 1 immunoreactivity in inflamed human bowel. Lancet, 2001, 357 (9265): 1338-1339.
  • 9Chan CL, Facer P, Davis JB, et al. Sensory fibres expressing capsaicin receptor TRPV1 in patients with rectal hypersensitivity and faecal urgency. Lancet, 2003, 361 (9355): 385-391.
  • 10Akbar A, Yiangou Y, Facer P, et al. Increased capsaicin receptor TRPVl-expressing sensory fibres in irritable bowel syndrome and their correlation with abdominal pain. Gut, 2008, 57 (7): 923-929.

二级参考文献6

共引文献10

同被引文献189

引证文献16

二级引证文献136

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部