内皮抑素联合微波消融治疗裸鼠肾癌移植瘤研究

Microwave ablation combined with endostatin in treatment of human kidney carcinoma transplanted in nude mice

目的研究内皮抑素联合微波消融对裸鼠肾癌移植瘤的抑制作用。方法将人源性肾癌细胞接种到48只小鼠的背部皮下,肿瘤直径约1.5 cm时随机分成4组,对照组:隔日尾静脉生理盐水;联合治疗组:隔日尾静脉推注内皮抑素+皮下肿瘤微波消融;微波消融组:皮下肿瘤微波消融;内皮抑素组:隔日尾静脉推注内皮抑素。首先对联合治疗组及微波消融组局部皮下肾移植瘤进行微波消融治疗,其后经尾静脉注射药物。14天后每组处死6只小鼠,比较各组瘤重,计算抑瘤率,检测血浆VEGF浓度,剩余6只小鼠观察其存活期。结果联合治疗组小鼠肿瘤体积显著小于其他各组(P<0.0 5),其血浆VEGF水平较其他各组显著减低(P<0.0 5),存活时间较其他各组显著延长(P<0.05)。结论内皮抑素联合微波消融抗肿瘤作用优于单用内皮抑素或微波消融治疗,并使小鼠存活期延长。

Objective To investigate microwave ablation combined with endostatin in treatment of human kidney carcinoma transplanted in nude mice.Methods Human kidney Ketr-3 kidney cells were subcutaneously implanted in 48 BalB/C-nu/nu mice.When the diameter of tumor reached 1.5 cm the animals were randomly divided into 4 groups： in microwave group the mice were treated with microwave ablation;in endostatin group endostatin was injected through tail vein;in combination group the mice received endostatin injection and microwave ablation;in control group normal saline was injected.The tumor weight and the inhibition rate were calculated,and the survival time was observed.Plasma concentrations of vascular endothelial growth factor（VEGF） of tumors were measured by ELISA method.Results The tumor weight was lower in the combined treatment group than in the other groups（P0.05）.Plasma VEGF in combined treatment group was significantly decreased compared with that of other groups（P0.05）.Conclusion Microwave ablation combined with endostatin shows better therapeutical effects than each method used alone in treatment of transplanted human kidney carcinoma in nude mice.