脾酪氨酸激酶与食管鳞状细胞癌的关系

Expression of spleen tyrosine kinase in human esophageal squamous cell carcinoma

目的:探讨脾酪氨酸激酶(spleen tyrosine kinase,Syk)在食管鳞状细胞癌组织中的表达及其临床意义。方法:采用逆转录-聚合酶链反应(RT-PCR)检测30例食管鳞癌组织、27例食管鳞癌癌旁组织及30例相对正常食管黏膜组织中Syk mRNA的表达;应用免疫组化技术检测39例食管鳞癌组织、27例食管鳞癌癌旁组织及38例相对正常食管黏膜组织中Syk蛋白的表达并分析其与临床病理学指标间的关系。结果:食管鳞癌组织中Syk mRNA表达高于癌旁组织和正常食管黏膜组织,但差异无统计学意义(P>0.05)。Syk蛋白在食管鳞癌组织中的阳性率(34/39,87.2%)明显高于食管鳞癌癌旁组织(18/27,66.7%)和正常食管黏膜组织(26/38,68.4%)(P<0.05)。Syk蛋白表达与食管鳞癌患者年龄、性别、肿瘤大小、临床分期均无明显相关关系(P均>0.05)。结论:Syk mRNA和蛋白在食管鳞癌组织中表达增高,但Syk蛋白表达与临床病理学指标无相关关系。

OBJECTIVE： To explore the expression and its clinical significance of Syk（spleen tyrosine kinase） gene in human esophageal squamous cell carcinoma（ESCC）.METHODS： The expressions of Syk gene in human esophageal carcinoma lesions（n=30）,peri-cancerous tissue（n=27） and relative normal tunica mucosa of esophageal tissue（n=30） were detected by Reverse transcription-polymerase chain reaction（RT-PCR） technique.Immunohistochemistry was used to detect Syk protein expression in the above cases and analyzed their relationships with clinicopathologic data of the patients.RESULTS： The differences of Syk mRNA levels among the human esophageal carcinoma lesions,peri-cancerous tissue and relative normal tunica mucosa esophageal tissue were not significant（P0.05）.But the level of Syk mRNA was obviously higher the carcinoma lesions than the normal esophageal tissue.Of the 39 esophageal carcinoma lesions,34 were Syk protein positive（34/39, 87.2%）,expression of Syk protein were detected in 18 peri-cancerous tissue（18/27,66.7%） and in 26 of the 38 relative normal tunica mucosa esophageal tissue（26/38,68.4%）.A significant difference was noted among three groups（P0.05）.Furthermore,Syk protein expression was not associated with the patients′ sex,age,tumor size and stages（P0.05）.CONCLUSION： Expressions of Syk mRNA and Syk protein in ESCC were significantly higher and unrelated to clinicopathologic characteristics.