HSV-1感染对神经胶质瘤细胞神经生长因子及其受体表达的影响

Effect of HSV-1 Infection on NGF and Its Receptor Expression in Human Glioma Cells

神经生长因子(NGF)主要由神经胶质细胞产生,通过特异的靶细胞表面的神经生长因子受体介导产生生物学效应,与神经细胞的生长发育、分化和凋亡等密切相关。单纯疱疹病毒1型(HSV-1)作为一种嗜神经病毒,易造成神经细胞、神经胶质细胞凋亡或死亡。本实验以U251人神经胶质瘤细胞为研究对象,观察HSV-1感染致U251细胞凋亡的过程中NGF及其受体的变化情况。结果发现U251细胞是HSV-1的容许细胞;HSV-1感染致U251细胞凋亡过程中,NGF及其低亲和力受体p75NTR出现表达强度随时间先增强后减弱的趋势,而高亲和受体Tr-kA持续低表达。推测HSV-1感染致神经细胞凋亡中可能调控了神经营养因子的表达。

Nerve growth factor（NGF） is mainly secreted by the neuroglia cells,which can exert biological effect through its receptors on the specific target cell surface.NGF is closely related to neurocyte growth,differentiation and apoptosis.As a neurotropic virus,HSV-1 an easily lead to neurocyte,neuroglia cells death or apoptosis.In this study,the U251 human glioma cells were chosen as target cells to study the change of NGF and its receptors in the apoptosis process of HSV-1 infection.Our results showed that U251 cells were permissive to HSV-1 replication.In the apoptosis process of HSV-1 infected U251 cells,the expression of both NGF and P75NTR increased and then decreased,while the expression of TrkA decreased gradually.These result indicated that HSV-1 was able to induce the abnormal expression of NGF and its receptors in U251 cells.