rSj26-Sj32-Immunogold-Dipstick试剂对慢性日本血吸虫病的诊断价值研究

The Study on Diagnostic Value of the Immunogold-Dipstick Reagent Developed with Sj26-Sj32 Recombinant Protein for Chronic Schistosomiasis japonica

目的研究rSj26-Sj32-Immunogold-Dipstick试剂对慢性日本血吸虫病的诊断价值。方法用rSj26-Sj32融合蛋白和日本血吸虫成虫抗原(SjAWA)Immunogold-Dipstick法检测慢性日本血吸虫病患者血清,同时以华支睾吸虫病、卫氏并殖吸虫病、泡型棘球蚴病、囊性棘球蚴病、乙型肝炎、肺结核患者和健康人血清作为对照,比较两种抗原检测抗体效果的差异。结果该法检测慢性日本血吸虫病的敏感性和特异性分别为92.50%和97.67%,诊断该病的阳性预告值、阴性预告值及诊断效率分别为97.37%、93.33%和95.18%,并且与其他疾病患者血清均无交叉反应。结论 rSj26-Sj32-Immunogold-Dipstick试剂可用于慢性日本血吸虫病的免疫诊断。

Objective To evaluate the diagnostic value of the Immunogold-Dipstick reagent developed with Sj26-Sj32 recombinant protein for chronic schistosomiasis japonica.Methods The Immunogold-Dipstick method with Sj26-Sj32 recombinant protein and Schistosoma japonicum adult worms antigen（SjAWA） were used for detecting IgG in sera of chronic schistosomiasis japonica patients,sera from the patients with clonorchiasis,paragonimiasis westermani,alveolar echinococcosis,cystic echinococcosis,hepatitis B,tuberculosis and healthy people were used as control.Results The sensitivity and specificity of this method were 92.50% and 97.67%,respectively.The positive predictive value,negative predictive value and diagnosis efficiency were 97.37%,93.33% and 95.18%,respectively.There was no cross reaction with sera of the mentioned parasitic diseases.Conclusion The recombinant Sj26-Sj32-Immunogold-Dipstick reagent may be used to immunodiagnosis of chronic schistosomiasis japonica.