HLA-A＊3101、B＊4001、B＊5801、DRB1＊1602 等位基因与遵义地区汉族肾综合征出血热发病的相关性研究

Association of HLA-A ＊ 3101, B ＊ 4001, B ＊ 5801 and DRB1 ＊ 1602 alleles with hemorrhagic fever with renal syndrome disease in Han population of Zunyi province

目的 探讨HLA-A＊31、B＊40、B＊58、DRB1＊16位点基因多态性与遵义地区汉族肾综合征出血热（HFRS）的关联性.方法 采用群体研究方法,应用聚合酶链反应-序列特异性引物（PCR-SSP）技术对100例HFRS患者（患者组）和100例健康对照者（健康对照组）进行HLA-A＊31、B＊40、B＊58、DRB1＊16基因亚型分型,比较基因频率（GF）,并计算其相对危险度（RR）.结果 HFRS患者组HLA-A＊3101、B＊5801、DRB1＊1602的基因频率均较对照组明显增高（RR=13.825,x2=4.296,P=0.038 RR=2.614,x2=6.133,P=0.013 RR=8.523,x2=8.865,P=0.003）,差异有统计学意义（P均＜0.05） 患者组HLA-B＊4001的基因频率较对照组明显降低（RR=0.414,x2=6.640,P=0.010）,差异有统计学意义（P＜0.05）.结论 遵义地区汉族人群中,HLA-A＊3101、B＊5801、DRB1＊1602等位基因与HFRS呈正相关,HLA-B＊4001等位基因与HFRS呈负相关.

Objective To explore the association between HLA-A＊31, B＊40, B＊58 and DRB1＊16 allele polymorphisms and onset of hemorrhagic fever with renal syndrome （HFRS） in Zunyi Han population. Methods Using group study, HLA-A＊31, B＊40, B＊58 and DRB1＊16 genotyping was conducted in 100 HFRS cases and 100 controls among Han population in Zunyi area with polymerase chain reactionsequence specific primer（PCR-SSP）, gene frequency （GF） and relative risk （RR） were calculated and compared. Results The results showed that the frequencies of A＊3101, B＊5801 and DRB1＊1602 were increased in patients as compared to the healthy controls （ RR = 13. 825, x2 = 4. 296, P = 0. 038 RR =2.614,x2 =6. 133,P=0.013 RR =8.523,x2 =8. 865,P=0. 003）. The frequency of B＊4001 in patients with HFRS were significantly lower than that in the healthy controls（ RR =0.414,x2 =6.640,P =0. 010）.Conclusion These results suggest that HLA-A＊3101, B＊5801 and DRB1＊1602 haplotypes were strongly associated with susceptibility to HFRS disease in Zunyi Han population and allele HLA-B＊4001 might be associated with protection against hantaviruses infection.