摘要
目的 探讨HLA-A*31、B*40、B*58、DRB1*16位点基因多态性与遵义地区汉族肾综合征出血热(HFRS)的关联性.方法 采用群体研究方法,应用聚合酶链反应-序列特异性引物(PCR-SSP)技术对100例HFRS患者(患者组)和100例健康对照者(健康对照组)进行HLA-A*31、B*40、B*58、DRB1*16基因亚型分型,比较基因频率(GF),并计算其相对危险度(RR).结果 HFRS患者组HLA-A*3101、B*5801、DRB1*1602的基因频率均较对照组明显增高(RR=13.825,x2=4.296,P=0.038 RR=2.614,x2=6.133,P=0.013 RR=8.523,x2=8.865,P=0.003),差异有统计学意义(P均<0.05) 患者组HLA-B*4001的基因频率较对照组明显降低(RR=0.414,x2=6.640,P=0.010),差异有统计学意义(P<0.05).结论 遵义地区汉族人群中,HLA-A*3101、B*5801、DRB1*1602等位基因与HFRS呈正相关,HLA-B*4001等位基因与HFRS呈负相关.
Objective To explore the association between HLA-A*31, B*40, B*58 and DRB1*16 allele polymorphisms and onset of hemorrhagic fever with renal syndrome (HFRS) in Zunyi Han population. Methods Using group study, HLA-A*31, B*40, B*58 and DRB1*16 genotyping was conducted in 100 HFRS cases and 100 controls among Han population in Zunyi area with polymerase chain reactionsequence specific primer(PCR-SSP), gene frequency (GF) and relative risk (RR) were calculated and compared. Results The results showed that the frequencies of A*3101, B*5801 and DRB1*1602 were increased in patients as compared to the healthy controls ( RR = 13. 825, x2 = 4. 296, P = 0. 038 RR =2.614,x2 =6. 133,P=0.013 RR =8.523,x2 =8. 865,P=0. 003). The frequency of B*4001 in patients with HFRS were significantly lower than that in the healthy controls( RR =0.414,x2 =6.640,P =0. 010).Conclusion These results suggest that HLA-A*3101, B*5801 and DRB1*1602 haplotypes were strongly associated with susceptibility to HFRS disease in Zunyi Han population and allele HLA-B*4001 might be associated with protection against hantaviruses infection.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2010年第11期1004-1007,共4页
Chinese Journal of Microbiology and Immunology
基金
基金项目:贵州省社会发展攻关计划项目[黔科合NY(2006)3048]
遵义医学院硕士科研启动基金(F-138)
关键词
人类白细胞抗原
基因亚型
肾综合征出血热
相关性
Human leukocyte antigen Gene haplotype Hemorrhagic fever with renal syndrome Association