副黏病毒Tianjin株缺损性干扰颗粒增强小鼠免疫应答的初步研究

Study on defective interfering particles of Paramyxovirus, Tianjin strain enhanced the immune response of the mice

目的 探讨副黏病毒Tianjin株缺损性干扰（DI）颗粒作为新型生物佐剂的可行性.方法 分离提纯副黏病毒Tianjin株DI颗粒并鉴定,和脊髓灰质炎病毒（PV）灭活抗原等量混合后给小鼠皮下多点注射,并设氢氧化铝凝胶佐剂组、无佐剂组以及空白对照组.于免疫后14 d摘眼球取血检测免疫血清PV特异性IgG 无菌取脾脏制备脾细胞悬液,分别用MTT法进行T、B淋巴细胞增殖试验以及检测脾淋巴细胞接受PV灭活抗原再次刺激后IFN-γ、IL-4的产生情况.结果 副黏病毒Tianjin株DI颗粒佐剂组T、B淋巴细胞刺激指数（SI）、脾淋巴细胞分泌IFN-γ的量与其他组相比较,差异有统计学意义（P＜0.05）.结论 副黏病毒Tianjin株DI颗粒具有一定的增强机体免疫应答能力的作用,且以诱导Th1型细胞免疫应答为主.

Objective To investigate whether the defective interfering（DI） particles of Paramyxovirus, Tianjin strain could be used as a candidate adjuvant. Methods DI particles were separated and purified. After being identified, it was equally mixed with the inactivated poliovirus （PV） vaccine. Kunming mice were administered subcutaneously with the mixture, besides we set groups of inactivated PV vaccine containing Al（OH） 3 as an adjuvant, inactivated PV vaccine of which the dose was doubled without any adjuvant, and negative control. Sera were collected in the 14th day after immunization, and the specific antibodies of PV were detected. T/B lymphocyte stimulation indexes（SI） were counted through the lymphocyte proliferation tests. The quantities of IFN-γ/IL-4 produced by the splenocytes which were stimulated again by PV antigen were detected. Results The SI and the quantity of IFN-γof the mice in the group of inactivated PV vaccine combining with DI particles of Paramyxovirus, Tianjin strain were more than other groups. Conclusion DI particles of Paramyxovirus, Tianjin strain could enhance the immune response of inactivated poliovirus, especially the cellullar immunologic response of Th1 type.