UPLC法同时测定苍耳子中4种酚酸类成分的含量

Simultaneous determination of 4 phenolic acids in Cangerzi by Ultra-performance liquid chromatography

建立苍耳子中原儿茶酸、新绿原酸、隐绿原酸、1,3-二咖啡酰奎宁酸的UPLC同时检测方法,测定和比较28批不同产地和集散地苍耳子中4种酚酸的含量。研究采用UPLC法的流动相为甲醇-0.1%磷酸水溶液,检测波长为220和327 nm,流速0.4 mL.min-1,柱温35℃。结果显示,原儿茶酸、新绿原酸、隐绿原酸和1,3-二咖啡酰奎宁酸分别在0.357 0～35.70、2.500～250.0、1.060～106.1和1.010～101.0μg.mL-1内具有良好的线性关系(r2=0.999 9),平均回收率分别为(n=6)97.68%、99.55%、97.92%和100.4%。该方法快速、准确、重复性好,可为苍耳子的质量控制提供快速准确的检测方法。

In this study,an analytical method was developed and used to quantify simultaneously protocatechuic acid,neochlorogenic acid,cryptochlorogenic acid and 1,3-dicaffeoylquinic acid — four bioactive compounds contained in Fructus Xanthii using UPLC.The contents of four phenolic components of 28 batches of samples collected from different product areas and markets were determined and compared by means of this established method.The mobile phase was composed of methanol and water containing 0.1% phosphoric acid.Chromatography was monitored at dual-wavelengths — 220 and 327 nm.Flow rate was 0.4 mL.min？1 and column temperature was 35 ℃.The correlation coefficient between concentration and chromatographic peak area of protocatechuic acid,neochlorogenic acid,cryptochlorogenic acid and 1,3-dicaffeoylquinic acid was over 0.999 9 in the range of 0.357 0？35.70,2.500？250.0,1.060？106.1,1.010？101.0 μg.mL？1,respectively.The average recoveries of the four compounds were 97.68%,99.55%,97.92% and 100.4%,respectively.In conclusion,the established method can rapidly attain an accurate and reproducible result used to control the quality of Fructus Xanthii.