结核性胸膜炎血清和胸水中IL-17的表达水平及其意义

The serum and chest fluid level and significance of IL-17 in patients with tuberculous pleural effusion

目的:探讨结核性胸膜炎血清和胸水中IL-17的表达水平及其临床意义。方法:收集住院收治的初治结核性胸膜炎患者30例和非结核性胸膜炎患者20例,另选择健康体检人员20例为正常对照组。采用双抗体夹心ELISA测定血清及胸水中IL-17的含量。结果:结核性胸膜炎组血清中IL-17的含量高于正常对照组和非结核性胸膜炎组(P<0.05),具有统计学意义。结核性胸膜炎组胸水中IL-17的含量高于非结核性胸膜炎组(P<0.05)。结核性胸膜炎组和非结核性胸膜炎组的胸水与对应血清中的IL-8的含量具有统计学差异(P<0.05)。结核性胸膜炎组患者在规则治疗1、3、7、14 d时,血清和胸水中IL-17的含量均逐渐下降,血清中IL-17的含量于治疗3 d时显著低于入院时水平(P<0.05),胸水中IL-17的含量于治疗7 d时显著低于入院时水平(P<0.05)。结论:IL-17参与了结核性胸膜炎的发病机制,可以作为早期诊断及观察治疗效果的分子学免疫指标。

AIM： To investigate the serum and chest fluid level of IL-17 in patients with tuberculous pleural effusion and its pathological role.METHODS： ELISA was used to measure the blood serum and chest fluid level of IL-l7 from 30 patients with tuberculous pleural effusion,20 other patients without tuberculous pleural effusion,and 20 healthy persons.RESULTS： The blood serum level of IL-l7 were higher in tuberculous pleural effusion than in the other group.The chest fluid level of IL-l7 in patients with tuberculous pleural effusion were significantly higher than those in patients without tuberculous pleural effusion（P0.05）.The chest fluid level of IL-l7 in patients with and without tuberculous pleural effusion were significantly higher than that of the blood serum level.After treatment for 1,3,7 and 14 days,the blood serum and chest fluid level of IL-l7 were obviously lower（P0.01）.After treatment for 3 days,the blood serum level of IL-l7 were obviously lower than before treatment（P0.01）.After treatment for 7 days,the chest fluid level of IL-l7 were obviously lower than before treatment（P0.01）.CONCLUSION： IL-l7 might play an important role in the pathogenesis of tuberculous pleural effusion and IL-17 can be as an index of molecular immunology to diagnosis early and judge the effect of therapeutic.