水稻导入系聚合派生耐旱株系的培育和导入遗传成份的分子检测

Development of Introgression-line Pyramiding-derived-lines and Molecular Survey of Introgressed Segements in Rice

以中413为轮回亲本,C418和粤香占为供体材料,分别培育导入系并进行抗旱筛选,入选株系互相杂交,培育导入系聚合派生株系(introgression-linepyramiding-derived-lines,ILPDLs),经过大田和根管栽培条件下的抗旱性重复鉴定,获得1对营养生长期对干旱胁迫具有强耐受力的姊妹系(KH168,KH172)。以典型旱稻品种IRAT109和轮回亲本中413为对照,KH168和KH172在大田干旱胁迫下能维持较高的相对含水量,在对照品种严重卷叶和大量叶片枯死情况下,KH168和KH172维持较好的叶片生长状态,卷叶和叶片枯死显著减低。利用351个多态SSR标记检测2个株系材料的基因型,发现KH168和KH172分别有81个和77个导入片段,在12条染色体上均有分布,大部分染色体上的导入遗传成份由2个供体亲本共同提供。

Introgression line（IL） populations were developed using Zhong 413 as the recurrent parent,C418 and Yuexiangzhan as donor,respectively.Introgression-line pyramiding-derived-lines（PDLs） were developed by crossing those selected introgression lines with drought tolerance.A pair of sib-lines（KH168 and KH172） having strong drought tolerance at vegetative stage,were screened out in field and PVC-tube experiments.Both KH168 and KH172 can maintain high relative water content under water deficit in comparison with the typical upland variety IRAT109（CK1） and the recurrent parent Zhong 413（CK2）.While both control varieties had severe leaf rolling and desiccation,two PDLs maitained better leaf growing status and had significantly less leaf rolling and desiccation.The two lines were genotyped using 351 polymorphic SSR markers,showing KH168 carried 81 introgression segments and KH172 carried 77 segments distributing on 12 chromosomes.In most cases,introgression components on each chromosome came from both donor parents.