摘要
耐低氮种质的发掘及其在高原粳稻中应用,有利于提升高原稻区的生产效率并保护环境。在云南高原稻区两种土壤氮素水平下,种植以育成的优质高原粳稻品种滇粳优1号(DJY1)为轮回亲本所形成的2064份导入系,以叶绿素计读数(SPAD)值为指标,评价导入系的耐低氮特性。结果显示:供试导入系的SPAD平均值在两种不同土壤氮素水平下均呈正态分布,且与有效穗和穗粒数极显著正相关;以超出95%右尾概率值为标准,初步筛选出供体亲本为Khazar(BC3F3)和Chhomrong(BC3F4)2个导入系为低氮钝感材料,即耐低氮材料;以小于5%左尾概率值为标准,则推论出供体亲本为Basmati370(BC3F3)、Type3(BC3F3)、Khole-Mavshi(BC3F3)、Ajaya(BC3F3)、Doddi(BC3F3)和鱼秋谷(BC4F3)的6个导入系为低氮敏感材料。这些材料可用于高原稻区耐低氮及氮高效育种及遗传研究。
The aim is to screen for germplasms with tolerance to low nitrogen and to apply them into Japonica rice production in the high plateau zone and the result is further helpful to increase production efficiency and protect the environment.A total of 2 064 introgression lines derived from Dianjingyou 1,an improved and released variety in Yunnan high plateau zone as recurrent parent were grown under two different soil nitrogen contents.They were evaluated in tolerance to low nitrogen by using SPAD value as criterion.As the results,SPAD mean values of the introgression line used showed normal distributions under both the two different soil nitrogen contents.Significant positive correlations were found between SPAD mean value and either effective panicle number or spike per panicle.Two introgression lines derived from Khazar(BC3F3) or Chhomrong(BC3F4) as donor parent,were preliminarily selected as low-nitrogen tolerant germplasms based on their SPAD mean values bigger than the 95% critical value in the one-tailed test.Six introgression lines derived from one among Basmati370(BC3F3),Type3(BC3F3),KholeMavshi(BC3F3),Ajaya(BC3F3),Doddi(BC3F3) and YuQiuGu(BC4F3) as donor parent were primarily considered as sensitive germplasm to low nitrogen based on their SPAD mean values less than the 5% critical value in the one-tailed test.The introgression lines screened out above could be applied into breeding for tolerance to low nitrogen or nitrogen high efficiency in Japonica rice production in the high plateau zone in the future.
出处
《分子植物育种》
CAS
CSCD
2010年第6期1166-1171,共6页
Molecular Plant Breeding
基金
农业部948重大研究项目(2006-G1)
国家863计划"绿色超级稻新品种培育"项目
国家"十一五"科技支撑(2006BAD13B01)
云南省人才培引项目(2008PY049)共同资助
关键词
SPAD值
耐低氮
低氮敏感型
种质
水稻
SPAD-value
Tolerance to low-nitrogen
Sensitive to low-nitrogen
Germplasm
Rice