果蝇非经典剪接位点的生物信息学预测

Bioinformatics Prediction of Non-Canonical Splice Sites in Drosophila melanogaster

目的:计算识别果蝇中新的非经典剪接位点,以探索未知的剪接机制。方法:基于黑腹果蝇表达序列标签(EST)与其基因组序列比对数据重构基因结构,从中发现非经典的剪接位点,并采用Weblogo软件分析非经典剪接位点上下游序列,以期发现剪接相关的特异性元件。结果:共得到265个非经典的剪接位点,这些剪接位点落在195个蛋白编码基因上。结论:应用生物信息学方法在果蝇中发现了上百个非经典剪接位点,为研究非经典剪接机制奠定了基础。

Objective： To analyze and predict non-canonical splice sites in Drosophila melanogaster,and to explore unknown splicing mechanisms.Methods： Based on the alignments between the genome sequences and ESTs,we predicted the gene structure and identified the non-canonical splice sites,and then analyzed the possible motifs in splicing process using Weblogo.Results： A total of 265 non-canonical splice sites were mapped to the fly genome,which fall on 195 protein-coding genes.Conclusion： The research discovered hundreds of non-canonical splice sites and laid the foundation for new mechanism of splicing.