电刺激大鼠小脑顶核对下丘脑神经元放电活动的影响

Effect of stimulation of fastigial nucleus on the firing activities of the hypothalamus neurons in rats

目的:研究电刺激小脑顶核(fastigial nucleus,FN)及微电泳Glu(glutamic acid)、MK-801对大鼠下丘脑(hypothalamus,HT)神经元自发放电活动的影响,探讨电刺激FN治疗脑卒中大鼠的机制。方法:应用细胞外记录的方法,记录电刺激FN及微电泳药物对HT神经元放电的影响。结果:电刺激频率为20Hz(低频)时,50%的HT神经元的放电频率增高(P<0.01),电刺激频率为100Hz(高频时),80%的HT神经元的放电频率增高(P<0.01);Glu对HT有紧张性兴奋作用,其拮抗剂MK-801能够明显抑制Glu诱致的兴奋作用;70%HT神经元在微电泳MK-801的基础上进行100Hz电刺激FN时,高频刺激诱发的兴奋作用被明显削弱。结论:采用电刺激FN治疗脑卒中,其可能机制是通过Glu的兴奋作用来调节HT的异常活动。

Objective：To research the effects of stimulation of fastigial nucleus（FN）,microelectrophoresis of glutamic acid（Glu） and MK-801 on the neuronal firing activities of the hypothalamus（HT） in rats and to explore the possible mechanism of FN in the treatment of stroke.Methods：To investigate the effect of stimulation of FN and microelectrophoresis of Glu and MK-801 on the spontaneous firing activities of HT neurons by using extracellular recording technigues.Results：The stimulation of the FN at low-frequency（20 Hz） could induce an exciting effect on the spontaneous activity of 50% of HT neurons in rats（P0.01）.With the increasing of stimulation frequency to 100 Hz,80% of HT neurons were excited（P0.01）.Glu showed catatonic exciting effect on the HT neurons,while MK-801 proderced an inhibition of Glu effect.During exposure to MK-801,stinulation of FN at 100 Hz did not show excitig effect in 70% of HT neurons.Conclusion：The possible mechanism of improving symptoms of stroke is through the stimulation of FN which modulates the activity of HT by exciting effect of Glu predominantly.