大鼠肌源性干细胞分化为神经样细胞过程中Hes1蛋白的表达差异

Expressional difference of Hes1 protein during the differentiation of muscle derived stem cells into neuron-like cells in rat

目的:研究Hes1蛋白在大鼠肌源性干细胞(MDSCs)体外诱导分化为神经样细胞过程中的表达差异。方法:大鼠乳鼠骨骼肌消化后体外培养增殖,第5代传代后细胞分为两组:对照组完全培养基培养,实验组加入诱导剂培养。6d后观察两组细胞的形态变化,并进行神经元特异性烯醇化酶(neuron-specific enolase,NSE)免疫细胞化学鉴定。然后通过免疫荧光和Western Blot技术观察Hes1蛋白的表达差异。结果:实验组细胞呈NSE染色阳性,对照组基本不表达;免疫荧光和Western Blot结果显示,与对照组比较,实验组Hes1蛋白表达较强(P<0.05)。结论:在体外,大鼠肌源性干细胞分化为神经样细胞过程中Hes1蛋白表达降低。

Objective：To investigate the differential expression of Hes1 protein during the proliferation and differentiation of muscle derived stem cells（MDSCs） into neuron-like cells in vitro.Methods：The muscle-derived stem cells from the neonate rat were cultured and purified clonally in vitro.The 5 th passage cells were divided into 2 groups.The control group was not intervened,only cultured by complete medium;and the experiment group was added with inductor.Morphological changes and neuron-specific enolase（NSE） immunocytochemistry were observed after six days.Immunofluorescence and Wescern Blot technigues were used to demonstrate the Hesl expressional difference.Results：The cells in the experiment group were observed NSE-immunoreactivities,but the control group was negative.Immunofluorescence and Western Blot showed that the expression level of Hes1 protein in the experiment group was much higher than that of the control group（P0.05）.Conclusion：In vitro,the expression of Hes1 protein is decreased when the MDSCs were induced into neuron-like cells.