HLA-A、B和DRB1流式微珠高分辨分型方法结果分析

Evaluation the efficacy of flow-beads high resolution reagents to identify HLA-A, B and DRB1

目的 分析流式微珠高分辨分型试剂检测样品HLA-A、B和DRB1的结果.方法 用美国one lambda公司流式微珠高分辨分型试剂对631例骨髓库样品进行HLA-A、B和DRB1三个位点的检测,计算用此试剂最终能得出高分结果 的比率.结果 631份样本中,三个位点均仅出现一组结果的只有3例,占0.475%.出现多组结果,但根据ASHI及NMDP标准能判为高分辨的,A位点有489例,占77.50%;B位点有520例,占82.41%;DRB1位点有582例,占92.23%;A、B和DRB1三个位点均能得出高分辨结果的有374例,占59.27%.结论 使用流式微珠高分辨试剂指定高分辨结果是可行的,大多数标本可获得高分辨结果.

Objective To assess the possibility of identifying HLA-A, B and DRB genotypes by flow beads high resolution reagents. Methods Flow-beads high resolution reagents were used to detect HLA-A,B and DRB1 alleles in 631 healthy people, and the proportion of high resolution results were calculated.Results Among 631 samples, only three（0. 475%）samples could be identified directly. However, we found by ASHI and NMDP standards that the distribution of HLA-A, B, DRB1 and all three loci were 489 （77. 50%） samples, 520（82.41%）samples, 582（92. 23%）samples and 374（59. 27%）samples respectively.Conclusion The flow-beads high resolution reagents could be used to analyze high resolution results in clinical settings.