豇豆抗锈病性苗期鉴定技术研究

Studies on the Techniques for Evaluation of Resistance of Cowpea Seedlings to Rust Disease

根据不同的接种方法、接种物浓度、保湿时间和温度对豇豆锈病（Uromycesvignae）苗期抗病性表现的影响，筛选出一套方便、可行的苗期抗病性鉴定技术：即在20～30℃温度下，用每视野含对个孢子（10×16倍）的夏孢子悬浮液于1～2片复叶时喷雾接种，保湿18～24h，接种后10～14d调查21个供试品种的病情指数，结果表明品种间抗病性差异显著．其中，益农红仁特长豆角表现免疫，金山长豆和成都紫荚白露表现为高抗．

A set of techniques suitable for evaluation of resistance of cowpea (Vigna sesquipdalis )seadlings to rust disease (Uromyces vignae) was established according to the dffects of different inocula-tion metheds, concentration of inocula, duration of keeping in saturated humidity, and temperatures on re-sistance of cowpea seedlines. In the conrete, the cowpea seedlings with the first or second Compoundleaves were sprayed with suspension containing 30 freshly-harvested uredospores in the field of a micro-scope (106×),and subsequently kept in saturated humidity for 18 to 24 h at 20 to 30 ℃. Disease In-dsxes (D,Is) based on the phenotypes of the leaves after inoculated for 10 ～14 d were calculated. Theresults fiom evaluation of resistance with the techniques showed that there were significant differences inresistance to the rust disease among 21 cowpea varieties tested. One of them, 'Yi nong hony ren te changdou jiao' was immune, and two, 'Jin shan chang dou,, and 'Chen du zi jia bai lu' were highly asistant.