大豆浓缩蛋白制备大豆多肽的研究

Production of Soybean Peptides Using Soy Protein Concentrate

本文以大豆浓缩蛋白为底物,采取纤维素酶与蛋白酶分步复合酶解法制取大豆多肽。经2%纤维素酶酶解,以高温高压作为预处理方法,碱性酶、中性酶、风味酶质量百分比例为3:1:1,pH为7.5,底物浓度为8%,酶解时间为5 h,经超滤后喷雾干燥,得到的大豆多肽粉末其大豆多肽含量为98.5%,相对分子质量≤3286,总氮含量为82.1%。

In this paper,soy protein concentrate was hydrolyzed by cellulose and protease for preparation of soybean peptide.The substrate was pre-hydrolyzed by 2% cellulase enzyme and pretreated by high temperature and pressure and then enzymatically hydrolyzed under the following optimized conditions： alkaline enzyme-neutral enzymes-flavor enzyme ratio of 3：1：1,pH of 7.5,substrate concentration of 8%,hydrolysis time of 5h.After the treatments of ultrafiltration and spray drying,the molecular weights of 98.5% soybean peptides were ≤ 3286 D,and total nitrogen content of soybean peptide powder was of 82.1%.