LC-MS/MS法分析清爽型黄酒中的嘌呤含量

LC-MS/MS Analysis of Purine Content in Light Chinese Rice Wine

嘌呤是引起痛风的关键因素,检测黄酒中的嘌呤含量具有重要意义。本研究建立了一种采用液相色谱串联质谱法(LC-MS/MS)测定黄酒中嘌呤含量的方法。结果表明,清爽型黄酒中鸟嘌呤为0.67 mg/L,次黄嘌呤为0.62 mg/L,腺嘌呤为0.82 mg/L,总含量为2.12 mg/L。LC-MS/MS法样品加标回收率在98.2%-100.6%之间,相关系数大于0.995,相对标准偏差为3.8%-5.2%。

A new LC-MS/MS method was developed to determine purine content in light Chinese rice wine.It was found that the contents of Guanine,Hypoxanthine and Adenine in qingshuang Chinese rice wine were 0.67 mg/L,0.62 mg/L and 0.82 mg/L,respectively.And the total purine content was 2.12 mg/L.The recovery of the samples was in the range of 98.2%~100.6%,and the R and RSD values were of 0.995 and 3.8%~5.2%,respectively.