降钙素受体基因AluⅠ多态性与绝经后妇女骨密度的关系

Association between the Alu Ⅰ polymorphism in the calcitonin receptor gene and bone mineral density in postmenopausal women

目的观察降钙素受体基因AluⅠ多态性与绝经后妇女骨密度及骨转换生化标志物的关系。方法 ①2007-01/2008-12从福州常住汉族人中随机检测绝经后妇女623例,均知情同意。②记录年龄、绝经年限、体质量指数和绝经后骨折情况。③双能X线骨密度仪检测正位第2～4腰椎、左侧股骨颈、大转子和Ward's三角区骨密度。④PCR-RFLP技术检测降钙素受体基因AluⅠ多态性。⑤用酶联免疫吸附法检测骨转换生化标志物(血清骨钙素、血清骨碱性磷酸酶、尿吡啶啉和尿脱氧吡啶啉)。结果591例合格受试者进入结果分析,年龄48～84岁,平均62.19±6.32岁。①降钙素受体基因AluⅠ多态性各基因型间骨密度比较差异不显著(P>0.05)。②降钙素受体基因AluⅠ多态性各基因型间血清骨钙素、血清骨碱性磷酸酶和尿脱氧吡啶啉比较差异不显著(P>0.05),TT型尿吡啶啉明显低于CC型、TC型,组间比较差异显著(P<0.05)。③降钙素受体基因AluⅠ多态性各基因型间骨质疏松症例数比较差异不显著(P>0.05)。④降钙素受体基因AluⅠ多态性各基因型间绝经后骨折比较差异不显著(P>0.05)。结论 降钙素受体基因AluⅠ多态性与绝经后骨质疏松症无明显关联,不能作为福州地区绝经后妇女骨质疏松的遗传标记。

Objective To study the association between the calcitonin receptor gene Alu I genetic polymorphism and bone mineral density and biochemical markers of bone turnover in postmenopausal women. Methods （1）Six hundred and twenty-three postmenopausal Han ethnic women in Fuzhou city were randomly selected from January 2007 to December 2008. They all signed the consent form. （2） Age, menopause duration, body mineral index and postmenopausal fracture were recorded. （3） Bone mineral densities of lumbar vertebrae 2-4, left femoral neck, the greater trochantor, and Ward＇s triangle zone were measured with dual energy X-ray absorptiometry. （4） The polymorphic regions were amplified using PCR followed by digestion with restriction enzymes Alu I , and analyzed electrophoretically. （5）The biochemical markers of bone turnover including serum bone glaprotein, bone alkaline phosphatase, urinary pyridinoline, and urinary deoxypyridinoline were detected with enzyme linked immunosorbent assays. Results Totally 591 cases, aged from 48 to 84,62. 19 ±6.32 years in average, were qualified for the analysis. （1） There were no significant differences in bone mineral density among genotypes of Alu I polymorphism sites （P 〉 0.05 ）. （2） There were no significant differences in serum bone glaprotein, bone alkaline phosphatase and urinary deoxypyridinoline among genotypes of Alu I polymorphism sites （P 〉 0. 05 ）. Urinary pyridinoline was lower in TT genotype group than that in CC and TC genotype group （ P 〈 0.05 ）. （3）There were no significantdifferences in the incidence of osteoporosis among genotypes of Alu I polymorphism sites （P 〉 0.05 ）. （4） There were no significant differences in the incidence of postmenopausal fracture among genotypes of Alu I polymorphism sites （P 〉 0.05）. Conclusions Alu I polymorphism of the calcitonin receptor gene was not associated with postmenopausal osteoporosis and it could not be regarded as genetic markers of osteoporosis in Fuzhou postmenopausal women.