摘要
目的检测IL-22在支气管哮喘患者血清中的表达,检测IL-22R1在人气道上皮细胞、气道平滑肌细胞、肺成纤维细胞中的表达,寻找IL-22作用的靶细胞。方法应用ELISA法检测36例支气管哮喘患者及20例正常对照者血清IL-22及IL-17的表达,同时检测36例患者肺通气功能,根据1秒率(FEV1/FVC)及FEV1占预计值的百分比将支气管哮喘患者分为支气管激发试验阳性组(17例)和支气管舒张试验阳性组(19例)。应用实时定量PCR检测人气道平滑肌细胞、人肺成纤维细胞、人气道上皮细胞IL-22R1 mRNA的表达,用免疫荧光以及蛋白质印迹法检测IL-22R1蛋白在上述3种细胞中的表达。结果支气管哮喘患者血清IL-22及IL-17水平与正常对照组相比差异无统计学意义,但支气管舒张试验阳性组患者血清IL-22和IL-17水平高于支气管激发试验阳性组(P<0.05)。IL-22R1mRNA及蛋白在上述3种细胞均有表达。结论 IL-22可能涉及支气管哮喘的发生发展过程,气道平滑肌细胞、肺成纤维细胞、人气道上皮细胞可能均是IL-22发挥作用的靶细胞。
Objective To examine the serum level of IL-22 in asthmatic patients and the expression of IL-22R1 in human airway epithelial cells,human airway smooth muscle cells,and lung fibroblasts,so as to explore the target cells of IL-22.Methods The serum levels of IL-22 and IL-17 in 36 asthmatic patients and 20 normal control subjects were measured by enzyme-linked immunosorbent assay (ELISA).And lung function of the asthmatic patients was assessed by Gaeger spirometry.According to the values of FEV1/FVC and FEV1%,the patients were divided into two groups:bronchodilation test positive group (19 patients) and bronchial provocation test positive group (17 patients).The expression of IL-22R1 mRNA in human airway epithelial cells,human airway smooth muscle cells,and lung fibroblasts was examined using real-time PCR,and IL-22R1 protein expression was detected by immunofluorescence staining and Western blotting analysis.Results There was no significant difference in serum IL-22 and IL-17 levels between the asthmatic patients and normal controls.The serum IL-22 and IL-17 levels in asthmatic patients positive for bronchodilation test was significantly higher than those positive for bronchial provocation test(P〈0.05).IL-22R1 mRNA and protein were detected in all the 3 types of cells.Conclusion IL-22 may be involved in the pathogenesis of asthma,and human airway epithelial cells,human airway smooth muscle cells,and lung fibroblasts may all be the target cells of IL-22.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2010年第12期1291-1295,共5页
Academic Journal of Second Military Medical University