垂体瘤转化基因RNA干扰对胶质瘤细胞化疗敏感性的影响

RNA interference of pituitary tumor transforming gene affecting the chemotherapeutic sensitivity of glioma cells

目的 观察垂体瘤转化基因（PTTG）RNA干扰对胶质瘤细胞对化疗药物敏感性的影响.方法 实验分4组：对照组：对胶质瘤U251细胞不进行任何处理;替莫唑胺（TMZ）组：胶质瘤U251细胞培养基中加入TMZ;PTTG shRNA转染组：PTTG shRNA片段转染胶质瘤U251细胞;PTTG shRNA联合TMZ组：TMZ作用于转染PTTG shRNA片段的胶质瘤U251细胞.MTT法检测细胞生长状况,流式细胞仪检测细胞凋亡情况.结果 MTT结果显示吸光度值在对照组、TMZ组、PTTG shRNA转染组、PTTG shRNA联合TMZ组分别为0.85±0.07、0.58±0.06、0.55±0.07、0.41±0.05,TMZ组、PTTG shRNA转染组、PTTG shRNA联合TMZ组细胞增殖抑制率分别为（31.56±5.51）%、（35.53±4.60）%、（51.49±6.74）%;PTTG shRNA组及TMZ组与对照组比较差异具有统计学意义（P＜0.05）,而PTTG shRNA联合TMZ组抑制细胞增殖更明显,与PTTG shRNA组及TMZ组比较差异有统计学意义（P＜0.05）.流式细胞仪检测结果显示,48 h时对照组凋亡率为（6.29±0.78）%,TMZ组为（33.61±4.88）%,PTTG shRNA组为（39.61±4.95）%,PTTG shRNA联合TMZ组为（66.23±7.60）%,PTTG shRNA组及TMZ组与对照组比较差异有统计学意义（P＜0.05）,而PTTG shRNA联合TMZ组的凋亡率较PTTG shRNA组及TMZ组明显增高,比较差异有统计学意义（P＜0.05）.结论 PTTGRNA干扰可以增强胶质瘤细胞对化疗药物的敏感性,提高化疗效果.

Objective To investigate the chemotherapeutic sensitivity of glioma cells influenced by RNA interference of pituitary tumor transforming gene （PTTG）. Methods The glioma U251 cell line was divided into normal control group, TMZ treatment group, PTTG shRNA infection group and PTTG shRNA combined with TMZ treatment group. Cells in the later 2 groups were treated with PTTG shRNA and PTTG shRNA combined with TMZ. MTT assay and flow cytometry were employed to detect the cell proliferation and apoptosis of U251 cell line, respectively. Results The outcome of MTT assay showed that the growth and survive abilities were influenced after treating with PTTG shRNA and PTTG shRNA combined with TMZ; the optical density （OD） value of the control group, TMZ group,PTTG shRNA treatment group and PTTG shRNA combined with TMZ treatment group were （0.85±0.07）,（0.58±0.06）, （0.55±0.07） and （0.41 ±0.05）, respectively. The inhibitory rate of cell proliferation in the TMZ treatment group, PTTG shRNA treatment group and PTTG shRNA combined with TMZ treatment group were （31.56±5.51 ）%, （35.53±4.60）%, （51.49±6.74）%, respectively; statistical significance between control group and both PTTG shRNA group and TMZ group was noted （P〈0.05）; and statistical significance between group PTTG shRNA combined with TMZ and both PTTG shRNA group and TMZ group was noted too. The apoptosis rate in the control group, TMZ group, PTTG shRNA group and PTTG shRNA combined with TMZ group were （6.29±0.78）%, （33.63±4.88）%, （39.61 ±4.95）% and （66.23±7.60）%, respectively, 48 h after the treatment; significant differences were found between the control group and both PTTG shRNA group and TMZ group （P〈0.05）; and statistical increased apoptosis rate in the PTTG shRNA combined with TMZ group was noted as compared that in the PTTG shRNA group and TMZ group （P〈0.05）. Conclusion PTTG RNA interference, by inhibiting the cell proliferation and inducing the apoptosis of glioma cells, up-regulates the chemotherapeutic sensitivity and improve the effectiveness of chemotherapy.