摘要
目的 观察3-硝基丙酸（3-NP）诱导的继发性肌张力障碍大鼠模型的行为学变化,以建立一个可靠的继发性肌张力障碍动物模型.方法 36只雄性SD大鼠按随机数字表法分为3组,每组各12只,前2组为实验组（低剂量组立体定向注射2000 nmol 3-NP至大鼠左侧尾壳核,高剂量组立体定向注射4000nmol 3-NP）,第3组为对照组（注射等体积生理盐水）.术后观察大鼠头部扭转和自主行为变化,平板实验观察大鼠通过时间及步幅.结果 实验组大鼠给药后开始出现姿势障碍,头部向同侧扭转,其中高剂量组扭转评分明显大于低剂量组,比较差异有统计学意义（P＜0.05）.平板实验结果显示：实验组大鼠通过时间较对照组明显延长,步幅较对照组明显变小,比较差异有统计学意义（P＜0.05）,且偏于木板一侧行走,症状随剂量增加而加重.对照组大鼠术后第2天基本恢复术前状态,无头部偏转,均顺利通过木板实验.结论 大鼠尾壳核注射3-NP可诱导产生稳定的继发性肌张力障碍动物模型.
Objective To observe the behavioral changes of rats with 3-nitropropionic acid （3-NP） induced secondary dystonia to set up a kind of reliable secondary dystonia models. Methods Thirty-six SD rats were equally divided into control group and low-dosage 3-NP treatment group （2000nmol） and high-dosage 3-NP treatment group （4000 nmol）. The 3-NP was injected into the left caudate putamen （CPU） in rats of the later 2 groups and sodium chloride solution was injected into the left CPU in rats of control group. Narrow board training was proceeded and camera was employed to record the dystonic posture and both the time and steps passing the board. Results As compared with the control group, 3-NP treatment groups had obvious ipsilateral dystonic posturing （P〈0.05）, and the scores of dystonic posturing in the high-dosage 3-NP treatment group were significantly higher than those of that in the low-dosage 3-NP treatment group （P〈0.05）. Narrow board experimental results indicated that the time was significantly extended and length of step was significantly shortened in rats of 3-NP treatment groups as compared with those in the control group （P〈0.05）; rats in the treatment groups walked towards the lateral side of the boared, these symptoms became much severe following the increase of 3-NP dosage.While rats in the control group had no dystonic posture, and they can go though the board without retention. Conclusion The 3-NP injecting into the CPU of rats can induce a kind of stable animal models with secondary dystonia.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2010年第12期1208-1211,共4页
Chinese Journal of Neuromedicine
基金
北京市科技新星基金（2008-B-43）
