菱叶红景天组织培养及植株再生

Tissue culture and plant regeneration of Rhodiola henryi

目的:探索菱叶红景天组织培养与植株再生技术并优化体外繁殖体系。方法:采用正交试验设计分别研究外植体种类、植物生长物质种类和浓度对菱叶红景天愈伤组织的诱导、丛生芽的产生以及生根诱导的影响,实验数据进行极差和方差分析。结果:影响菱叶红景天愈伤组织诱导的因素依次为外植体>2,4-D>6-BA,叶片产生愈伤组织最佳的培养基为MS附加2,4-D 1.5 mg.L-1和6-BA0.5 mg.L-1;影响菱叶红景天丛生芽诱导的因素依次为外植体>NAA>6-BA,茎段产生丛生芽最佳的培养基为MS附加6-BA1.5 mg.L-1和NAA1.0 mg.L-1;试管苗生根的最佳培养基为1/2MS附加IBA1.0 mg.L-1,生根率可达90%以上,生根试管苗移栽成活率达98%以上。结论:本试验初步建立了菱叶红景天组织培养与植株再生的基本体系。

Objective： To study the tissue culture and plant regeneration technologies and optimizing propagation system in vitro of Rhodiola henryi. Method： Orthogonal experiment designs were used in the study of Rh. henryi callus induction, shoot formation and rooting, and the data were analyzed by range analysis and variance analysis. Result： The optimal media to induce muhiple callus from leaves were MS supplemented with 2,4-D 1.5 mg·L^-1 and 6-BA 0. 5 mg·L^-1, the effect of the three factors was in sequence of explants 〉 2,4-D 〉 6-BA; The optimal media to induce multiple buds from stems were MS supplemented with 6-BA 1.5 mg·L^-1 and NAA 1.0 mg·L^-1, the effect of the three factors was in sequence of explants 〉 NAA 〉 6-BA ; Plantlets were rooted on 1/2MS supplemented with IBA 1.0 mg·L^-1 , and rooting rate reached to 90% or more and transplant survival rate of plantlet reached 98% or more. Conclusion： An efficient system for tissue culture and plant regeneration of R2t. henry/was initially established.