HPLC同时测定泽泻中4种泽泻醇成分的含量

Simultaneous determination of four alisols in Rhizoma Alismatis by RP-HPLC

目的：建立同时测定泽泻药材中泽泻醇A、泽泻醇F、24-乙酰泽泻醇A和23-乙酰泽泻醇B含量的HPLC方法。方法：采用RP-C18色谱柱（6.0 mm×150 mm,5μm）,乙腈-水梯度洗脱,检测波长210 nm,正交实验优化药材提取方法并测定18个不同批次样品的含量。结果：泽泻醇A、泽泻醇F、24-乙酰泽泻醇A和23-乙酰泽泻醇B在测定的范围内均表现出良好的线性（r〈0.999）;方法的回收率在96.67%99.61%,RSD均小于3.0%。结论：该分析方法能简便快速的测定泽泻中多个主要成分的含量,可以较全面的反映泽泻药材质量的优劣。

Objective： To develop an HPLC method for the determination of alisol A,alisol F,alisol A 24-acetate and alisol B 23-acetate in the rhizome of Alisma orientalis.Method： The separation was performed on a Shim-Pack CLC-ODS a C18 column（6 mm×150 mm,5 μm） eluted with the mobile phases of acetonitrile（A）-water（B） in gradient elution.The absorbance was monitored at 210 nm.Orthogonal test was adopted to optimize the extraction conditions of the four alisols.Result： The correlation coefficients of the four alisols were higher than 0.999 and the average recoveries were 99.23%,96.67%,97.30% and 99.61%,respectively.All the RSDs were less than 3%.Conclusion： The validation data demonstrated that the method was accurate and repeatable,and can be use to measure the four alisols in Rhizoma Alismatis.