绞股蓝对光老化皮肤组织的影响

Effects of G. Pentaphyllumon on photoaging skin in nake mice

目的探讨外用绞股蓝对紫外线照射引起的无毛小鼠光老化模型皮肤组织中过氧化氢酶（CAT）、谷光甘肽过氧化物酶（GSH—PX）活性和羟脯氨酸（HYP）含量的变化及其对光老化皮肤组织的影响。方法将昆明种无毛小鼠随机分成5组，制模同时，给药组外用绞股蓝。测定其皮肤组织中CAT、GSH—PX活性及HYP含量。结果模型组CAT、GSH—PX活性明显降低，HYP含量明显减少（分别为P〈0．01，P〈0．05，P〈0．05），给药组中各指标均有不同程度的改善。结论绞股蓝可以通过提高CAT、GSH—PX的活性，增加HYP含量达到抗皮肤光老化的作用。

Objective To investigate G. Pentaphyllum＇s influence on the activity of glutathione peroxidase （GSH-PX） and eatalase （CAT） in the skin of hireless photoaging mice, as well as the content of hydroxyproline （HYP）. At the same time, G. Pentaphyllum＇s effect on the hireless photoaging mice was studied. Methods Healthy KM hireless mice were randomly divided into five groups. A skin photoaging model was established by G. Pentaphyllum administration. The changes of the activity of GSH-PX, CAT and the contents of dermal hydroxyproline in the skin were then measured by biochemical methods in each group of the experiment. Results In the model group, the activity of GSH- PX, CAT and the content of HYP of skin were all decreased significantly （P〈0.05）. And there were different degrees of improvements in those of the herb given groups. Conclusion G. Pentaphyllumhas the protective effect on the skin of the photoageing in the hairless mice. The mechanism may be that G. Pentaphyllum could inhibit the oxidative damages of ultraviolet and Skin photoaging by increasing the synthesis of collagen in skin.