HPLC测定多维生素糖浆中D-生物素的含量

Determination of D-biotin in alvityl syrup by HPLC

目的：建立HPLC-UV法测定多维生素糖浆中D-生物素的含量。方法：样品用混合溶剂（丙酮：己烷：乙酸乙酯=1：2：1）萃取后,采用HPLC-UV法测定其中D-生物素的含量,使用Agela AQ-C18柱（250 mm×4.6 mm,5μm）,流动相为5 mmol·L^-1己烷磺酸钠溶液（以0.02 mol·L^-1磷酸调pH至2.3）-乙腈（92∶8）;流速为1.0 mL·min^-1;检测波长为200 nm。结果：D-生物素的浓度在1-10μg·mL^-1范围内与峰面积线性关系良好（r=0.999 9,n=7）;平均回收率为100.7%（RSD=1.4%,n=6）。结论：本实验所建立的HPLC-UV分析方法快速、灵敏,色谱峰分离度良好,结果准确可靠,可作为多维生素糖浆的质量控制项目之一。

Objective：To develop an HPLC-UV method for the determination of D-biotin in alvityl syrup.Methods：After being extracted with mixed solution（acetone∶hexane∶ethyl acetate=1∶2∶1）,samples were separated by HPLC on an AQ-C18 column（250mm×4.6mm,5μm） with mobile phases of 5mmol·L^-1 sodium hexanesulfonate water solution（adjusted pH to 2.3 with 0.02mol·L^-1 phosphoric acid）-acetonitrile（92∶8）.The flow rate was 1.0mL·min^-1.The detection wavelength was 200nm.Results：Calibration curve for D-biotin was linear in the range of 1-10μg·mL^-1（r=0.9999,n=7）.The average recovery was 100.7%（RSD=1.4%,n=6）.Conclusion：This assay is sensitive,accurate and convenient with good resolution.It can be applied to control the quality of alvityl syrup.