期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

基于计算机模拟的蛋白质分离纯化实验

Experiment of Protein Purification Based on Computer Simulation
原文传递
导出
摘要 利用数学建模的方法首次提出了衡量分离纯化效果优劣的定量指标——纯化效益;建立了便于应用推广的聚乙二醇(polyethyleneglycol,PEG)和硫酸铵沉淀分离纯化模型,并以海芋过氧化物酶分离纯化为例,对模型进行求解、应用和检验,求解最优实验试剂用量. In this paper,we created the concept of effectiveness of purification to measure the quality of the protein purification by the mathematical modeling methods;extablished the protein purification model of polyethylene glycol and ammonium sulfate that easily to be used; and applied them on the purification of the Alocasia peroxidase,chacking the model,to find out the best amount of the reagents used in the experiment.
作者 刘文彬 张鹏 占磊 何平
机构地区 华南农业大学生命科学学院
出处 《生物数学学报》 CSCD 北大核心 2010年第3期493-500,共8页 Journal of Biomathematics
基金 广东省自然科学基金项目(7006655) 广东省科技计划项目(2008B011000006) 国家自然科学基金项目(50977041)
关键词 纯化效益 计算机模拟 过氧化物酶 Effectiveness of purification Computer simulation Peroxidase
分类号 Q503 [生物学—生物化学]
  • 相关文献

参考文献13

  • 1loannis V P, Dimitrios G, George K P, et al. Lipases in water-in-ionic liquid microemulsions: Structural and activity studies[J]. Journal of Molecular Catalysis B: Enzymatic, 2009, 60(1): 50-56.
  • 2Becker J S, Thomas O R T, Franzreb M. Protein separation with magnetic adsorbents in micellar aqueous two-phase systems[J]. Separation and Purification Technology, 2009, 6(1): 46-53.
  • 3壬建龙,文湘华.现代环境生物技术[M].北京,清华大学出版社,2001,46.
  • 4Stefano G, Elena B, Ilaria B,et al.Respiration, hydrogen peroxide levels and antioxidant enzyme activities during cold storage of zucchini squash fruit[J]. Postharvest Biology and Technology, 2009, 52(1): 16-23.
  • 5Bram L H M S, Henk A S, Martien A G S, et al. Influence of the overall charge and local charge density of pectin on the complex formation between pectin and β-lactoglobulin[J]. Food Hydrocolloids, 2009, 23(3):765- 772.
  • 6He J S, Ruan K C. Kinetics of phase separation during pressure-induced gelation of a whey protein isolate[J]. Food Hydrocolloids, 2009, 23(7):1729-1733.
  • 7Luisa A F, EugSnia A M, Simao P P. The effect of ammonium sulfate on the solubility of amino acids in water at (298.15 and 323.15) K[J]. The Journal of Chemical Thermodynamics, 2009, 41(2):193-196.
  • 8张尚立,刘刚.带不完全椭球约束生长曲线模型线性估计的泛容许性[J].生物数学学报,2008,23(1):160-164. 被引量：3
  • 9Ramesh D, Arthur V C, Robert B,et al. Silk-elastinlike protein polymer hydrogels: Influence of monomer sequence on physicochemical properties[J]. Polymer, 2009, 50(2):366-374.
  • 10Liao S J. Notes on the homotopy analysis method: Some definitions and theorems[J]. Communications in Nonlinear Science and Numerical Simulation, 2009, 14(4):983-997.

二级参考文献31

  • 1张双林.带约束线性模型中的可容许线性估计[J].应用数学学报,1994,17(3):470-472. 被引量：15
  • 2李永慈,唐守正.度量误差对模型参数估计值的影响及参数估计方法的比较研究[J].生物数学学报,2006,21(2):285-290. 被引量：12
  • 3Lindley D , Smith A. Bayes estimates for the linear model[J]. Journal of the Royal Statistical Society B,1972,34(1): 1-41.
  • 4Tsutakawa R K. Mixed models for analyzing geographic variability in mortality rates[J]. Journal of the American Statistical Association , 1988,83(1): 37-42.
  • 5Goldstein H. Multilevel mixed linear models analysis using iterative generalized least squares[J]. Biometrika, 1986,73(1): 43-56.
  • 6Longford N. Afast scoring algorithm for maximum likelihood estimation in unbalanced mixed models with nested random effects[J].Biometrika, 1987,74(4): 817-827.
  • 7Linstrom M J, Bates D M. Newton-Raphson and EM algorithms for linear mixed effects models for repeated measures data[J].Journal of the American Statistical Association, 1988,83(4): 1014-1022.
  • 8McCulloch C E. Maximum likelihood algorithms for generalized linear models[J]. Journal of the American Statistical Association, 1997,92(1): 162-170.
  • 9Zhu H T , Lee SY. Analysis of generalized linear mixed models via a stochastic approximation algorithm with Markov chain Monte-Carlo method[J].Statistics and Coumputing, 2002,12(2): 175-183.
  • 10Breslow, N. E. and Clayton, D. G. Approximate inference in generalized linear mixed models[J]. Journal of the American Statistical Association, 1993,88(1): 9-25.

共引文献8

生物数学学报
2010年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部