布尔山羊精液保存条件的试验研究

The Study on Keeping Sperm Trial in Boer Goats

本研究对不同条件下，用不同成分的稀释液保存布尔山羊精液的效果进行了对比试验。试验证明，选用Ａ液（３０ｇ／Ｌ葡萄糖、１３ｇ／Ｌ柠檬酸钠和１ｇ／Ｌ乙二胺四乙酸钠），按５０ｍＬ／Ｌ加入卵黄，稀释布尔山羊精液，在常温（１５～２１℃）和低温（４℃）条件下分别保存３０ｈ和９６ｈ，其活力分别为０．５０和０．５２，可完全满足输精要求；选用Ｂ液（５４ｇ／Ｌ葡萄糖和１０ｇ／Ｌ柠檬酸钠），进行细管冷冻保存布尔山羊精液，冻后精子的活力为０．５６；Ｂ液中分别按２．５ｇ／Ｌ添加ＶＥ或按２５ｍｍｏｌ／Ｌ添加ＶＣ，其冻后活力分别达到０．５８和０．６０。Ａ液在常温和低温条件下保存精液的效果优于Ｂ液，而Ｂ液的冷冻效果优于Ａ液。

In different conditions, the comparing trial of keeping sperm in Boer Gaots was studied. A solution contains 30 g/L glucose, 13 g/L sodium citrate and 1 g/L editic acid (EDTA). Sperm were diluted with A solution containing 50 mL/L egg yolk,on 15￣21 for 30 h, its viability is 0.50; on 4 for 96 h, it viability is 0.52. B solition contains 54 g/L glucose and 10 g/L sodium citrate. Boer Goats sperm in the straw were frozen with B solution, of Which the viability is 0.56; sperm in straw were frozen with B solution containing 2.5 g/L VE,its viability is 0.58; sperm in straw were frozen B solution containing 25 mmol/L VC, its viability is 0.60. on 15￣21 and 4 conditions, the result of keeping sperm with A solutin is better than B solution; the result of freezing sperm with B solition is better than A solutiobn