饲粮硒来源及添加水平对仔猪组织中细胞内谷胱甘肽过氧化物酶基因mRNA表达的影响

Effects of Sources and Levels of Dietary Selenium on the Expression Level of mRNA of Celluar Glutathione Peroxidase Gene in Piglets

本文旨在研究饲粮中硒来源及添加水平对仔猪细胞内谷胱甘肽过氧化物酶(GPX1)mRNA表达的影响,为合理利用各种硒源提供一定的理论基础。选择35日龄体重相近的三江白猪仔猪90头,随机分为10组,每组3个重复,每个重复3头猪。分别在9个试验组饲粮中添加纳米硒、酵母硒、亚硒酸钠,其剂量以硒计,添加量分别为0.1、0.3、0.5 mg/kg 3个水平,对照组饲粮中不另添加硒。试验期为35 d,试验结束后采用反转录RT-PCR技术测定仔猪肝脏、肾脏、脾脏、肌肉组织中GPX1 mRNA的表达量。结果表明:1)在肝脏组织中,0.5 mg/kg纳米硒组GPX1 mRNA表达量显著高于亚硒酸钠组(P<0.05),0.5 mg/kg纳米硒组和0.3 mg/kg酵母硒组显著高于对照组(P<0.05);2)在肾脏组织中,各试验组之间GPX1 mRNA表达量差异均不显著(P>0.05);3)在脾脏组织中,0.5 mg/kg纳米硒组GPX1 mRNA表达量显著高于酵母硒组和亚硒酸钠组(P<0.05),0.5 mg/kg纳米硒组和0.3 mg/kg酵母硒组显著高于对照组(P<0.05);4)在肌肉组织中,0.5 mg/kg纳米硒组GPX1 mRNA表达量显著低于亚硒酸钠组和对照组(P<0.05)。由此可见,在饲粮中添加0.5 mg/kg纳米硒能够提高仔猪肝脏、脾脏中GPX1 mRNA的表达量,而肌肉组织中的表达水平则低于亚硒酸钠组和对照组。

This experiment was conducted to study the effects of sources and levels of dietary selenium on the expression level of mRNA of celluar glutathione peroxidase gene in piglets,and to provide a theoretical basis for the rational application of various selenium sources.Ninety Sanjiang White piglets of 35 days old with similar body weight were selected in the experiment.They were randomly divided into 10 groups with 3 replicates per group and 3 piglets per replicate.Piglets in the 9 experimental groups were fed nano-selenium,yeast selenium and sodium selenite at 0.1,0.3 and 0.5 mg/kg（by selenium content）,respectively.No extra selenium was added to the diet of the control group.The experimental period was 35 days.RT-PCR was used to determine the expression level of mRNA of GPX1 gene in the liver,kidney,spleen,and muscle tissues of the piglets.The results showed as follows： 1） in the liver tissue,the expression level of GPX1 mRNA in nano-selenium group was significantly higher than that in sodium selenite group at 0.5 mg/kg addition level（P0.05）,and the expression level in 0.5 mg/kg nano-selenium group and 0.3 mg/kg yeast selenium group was significantly higher than that in the control group（P0.05）;2） in the kidney tissue,the differences of the expression level of GPX1 mRNA in all experimental groups were not significant（P0.05）;3） in the spleen tissue,the expression level of GPX1 mRNA in the nano-selenium group at 0.5 mg/kg was significantly higher than that in the sodium selenite group and the yeast selenium group（P0.05）,and the expression level in 0.5 mg/kg nano-selenium group and 0.3 mg/kg yeast selenium group was significantly higher than that in the control group（P0.05）;4） in the muscle tissue,the GPX1 mRNA expression level in the nano-selenium group at 0.5 mg/kg was significantly lower than that in the sodium selenite group and the control group（P0.05）.It is concluded that dietary addition of 0.5 mg/kg nano-selenium can increase the expression level of GPX1 mRNA in the liver and spleen tissues of piglets,but in the muscle tissue,the expression level was lower than that in the sodium selenite group and the control group.