YB-1在青年型乳腺癌组织中的表达及意义

The expression and significance of YB-1 in breast cancer of young patients

目的:探讨YB-1(Y-box binding protein 1)在青年型乳腺癌组织中的表达及意义。方法:应用免疫组织化学方法研究YB-1蛋白在青年型乳腺癌组织中的表达,并结合病理组织学类型、病理分级等临床特征分析YB-1在青年型乳腺癌组织中的表达意义;依据ER、PR、HER-2、CK5/6表达对青年型乳腺癌进行分子分型(Luminal-A、Luminal-B、HER2+和Basal-like 4个分子亚型),并分析其与YB-1表达的关系。结果:HE结果显示64例青年型乳腺癌病理组织学分类主要为浸润性导管癌,占59.4%。免疫组化结果显示64例青年型乳腺癌组织中YB-1阳性率为92.2%,其中细胞核阳性表达率53.1%,细胞浆阳性表达率39.1%。肿瘤>2cm的患者占59.4%,YB-1主要表达在细胞核(40.6%),患者肿瘤?2cm占37.5%,YB-1则主要在细胞浆表达(20.3%),YB-1表达类型与肿瘤大小有显著统计学意义(P=0.036)。组织学3级所占比例最高,占48.4%,且多为YB-1细胞核表达(37.5%),乳腺癌不同的组织学分级间YB-1的表达率不同(P=0.000);青年型乳腺癌患者发生淋巴结转移的比例高(84.4%),其中N3期所占比例最高,占40.6%,YB-1主要在细胞核表达,占31.3%,胞浆表达占7.8%。青年型乳腺癌分子分型中主最多见的是Luminal-B型,占46.9%,YB-1主要表达在细胞核(32.8%),浆表达仅占9.4%,统计分析显示YB-1表达的核浆分布特点与青年型乳腺癌分子分型有关(P=0.04)。结论:YB-1在青年型乳腺癌组织中的表达特点与肿瘤大小、病理组织分级及淋巴结状态有一定关系,细胞核阳性主要见于肿瘤>2cm的患者,而细胞浆阳性主要见于肿瘤?2cm的患者,且YB-1细胞核表达主要为组织学3级,且青年型乳腺癌淋巴结转移率高,YB-1主要在细胞核表达;青年型乳腺癌不同分子分型中YB-1蛋白表达存在差异,YB-1可能成为青年型乳腺癌防治的新的分子靶点。

Objective：To investigate the expression and significance of YB - 1 protein in breast cancer of young patients. Methods ：The expression of YB - 1 in breast cancer of young patients were studied by immunohistochemistry （IHC） ;The relationship between YB - 1 expression and significance with clinical characteristics such as histologic type, histopathologic grading, tumor size and lymph node status were analyzed; Four different molecular subtypes of breast cancer（ Luminal - A, Luminal - B, HER - 2 and Basal - like） were defined by IHC markers of estrogen recep- tor （ER）, progesterone receptor （PR）, HER2 and CK5/6. And the relationship between the expression of YB -1 and the molecular subtypes of breast cancer was also analyzed. Results： The positive rate of YB - 1 were 92.2% （ 59/ 64）, among which nucleus staining rate were 53. i % （ 34/64 ） , cytoplasm staining rate were 39. 1% （ 25/64 ）. When tumor size 〉2cm ,YB - 1 was mainly stained in nucleus （40.6%） ; while the tumor size ~ 2cm, YB - 1 was mainly stained in cytoplasm （20.3%）. There were significant correlation between YB -1 expression paterns and tumor size （ P = 0. 036）. In all eases of breast cancer the main histopathologie type was grade 3. Interestingly, in this group YB - 1 stained mainly in nucleus （ 3 7.5 % ）. There were significant different expression paterns of YB - 1 in different histopathologic grade of breast cancer （ P = 0. 000 ）. There was higher metastasis rate of lymph node （84.4%） among these young breast cancer patients. Among all 64 young breast cancer patients, the subtype of Luminal- B was the majority （46.9%） and the next were Luminal- A （25.0%）. YB- 1 primarily expressed in nucleus （32.8%） in the subtype of Luminal - B and cytoplasm expression was only 9.4%. There were different ex- pression paterns of YB - 1 in different molecular subtypes （ P = 0.04 ）. Conclusion： The expression paterns of YB - 1 in young breast cancer were related with the histopathologic characteristics such as tumor size, histopathologic grading and lymph node status. There were different kinds of expression of YB - 1 among different molecular subtypes in young breast cancer. YB - 1 can be used as a new molecular target in the prevention and therapy in young breast cancer.