凋亡抑制因子Livin在肺癌组织和血清中的表达

Expression of apoptosis inhibitor Livin in lung tissue and serum

目的:探讨凋亡抑制蛋白Livin在非小细胞肺癌组织和血清中的表达及其与组织类型、临床分期的关系。方法:采用免疫组化法和酶联免疫吸附法(ELISA)分别对40例肺癌组织和血清,20例癌旁组织和良性病变组织,20例健康者血清中Livin的表达水平进行检测。结果:非小细胞肺癌患者组织和血清中Livin水平明显高于对照组(P<0.05);组织中Livin蛋白的表达与淋巴结转移有关,但与肺癌临床分期、病理类型、分化程度无关。血清中Livin水平与淋巴结转移、临床分期有关,但与病理类型、分化程度无关;组织和血清间Livin水平无相关性。结论:Livin作为一种新的凋亡抑制蛋白,对非小细胞肺癌诊断、淋巴结转移的判断具有一定的临床价值。

Objective ： To study the expression of inhibitor of apoptosis protein Livin in non - small cell lung canc- er （NSCLC） in tissue and serum and relation to histological type, clinical stage. Methods ： The expression of Livin in 40 cases of NSCLC tissue and serum, 20 cases of adjacent normal tissue and benign lesions, 20 cases of healthy serum were detected by immunohistochemistry and enzyme -linked immnnosorbent assay （ELISA）. Results： Non - small cell lung cancer tissue and serum levels of Livin significantly higher （ P 〈 0.05 ） ; in the organization Livin pro- tein expression was related to lymph node metastasis, but not to clinical stage, histological type, histological grade. Livin in serum was related to lymph node metastasis,bat not to clinical stage, tumor type,tumor differentiation;In tissue and serum there was no correlation. Conclusion：Livin as a new inhibitor of apoptosis protein is useful for non - small cell lung cancer diagnosis and staging.