p53基因在骨肉瘤中的表达及其临床意义

Protein expression of p53 in osteosarcoma and the correlation with clinical features and prognosis

目的:探讨p53基因在骨肉瘤组织中的表达及其临床意义。方法:应用免疫组化方法(S-P法)测定p53蛋白在36例骨肉瘤和17例骨软骨瘤组织中的表达,并随访骨肉瘤患者生存时间。36例骨肉瘤中男19例,女17例,年龄平均19.9岁。Ennek ing分期Ⅱa3例,Ⅱb27例,Ⅲb6例,并发病理性骨折7例。结果:36例骨肉瘤随访时间平均3年6个月,其中存活3年及以上者13例,存活3年以下者23例,死亡病例均因骨肉瘤远处转移全身衰竭致死。36例骨肉瘤p53蛋白阳性表达率为52.8%,显著高于骨软骨瘤组,有显著性差异(P<0.01)。p53蛋白表达在不同Ennek ing外科分期组间无显著性差异(P>0.05),在生存时间3年以上组及3年以下组间有显著性差异(P<0.01)。结论:p53基因突变对骨肉瘤的发病可能产生影响,对患者预后评估具有一定价值。

Objective：To explore the protein expression of P53 in osteosarcoma and its relationship with clinical features and prognosis. Methods： The protein expression of P53 was detected by using immunohistochemistry （LSAB） in 36 cases of osteosarcoma and 17 cases of osteochondroma, proved by surgery and pathology, and the sur- vival time in 36 cases of osteosarcoma were followed - up. Results ： All 36 cases of osteosarcoma were followed - up with the average time of 42 months ,there were 13 cases survival more than 36 months, the positive expression rate of p53 was 52.8% in 36 cases of osteosarcoma, and higher than in osteochondroma , （P 〈0.01 ）. the positive expres- sion rate of p53 was higher in 23 cases survival less than 36 months. Conclusion：Threr is a definite relationship between p53 and osteosarcoma, p53 is useful for the assessment of the prognosis of osteosareoma.