钩藤碱对缺血-再灌注大鼠脑NOS变化的作用

Effect of rhynchophylline on the change of NOS in cerebral ischemia-reperfusion rats

目的 :研究钩藤碱 (Rhy)对大鼠脑缺血 -再灌注皮层和海马一氧化氮合酶 (NOS)变化的作用。方法 :阻断大鼠双侧颈总动脉 1 5min和再灌注 2 4h建立脑缺血 -再灌注损伤模型 ,用NADPH -d组化技术检测脑NOS阳性细胞数目的变化和Rhy的影响。结果 :脑缺血 -再灌注 2 4h后 ,皮层和海马NOS阳性细胞数目显著增多 ,Rhy( 1 2 .5和2 5mg/kg)缺血前 3 0min腹腔注射 ,可显著抑制此增多 ,与NOS拮抗剂L -NAME 1 0mg/kg的作用相似。结论 :Rhy能降低脑内NOS的活性 。

OBJECTIVE:To study the effect of rhynchophylline(Rhy) on the change of NOS activity in cortex and hippocampus in a rat ischemia-reperfusion model.METHOD:Cerebral ischemia-reperfusion of rats was induced by bilaterally occlusion of common cartid arteries for 15min and reperfusion for 24h.NADPH-diaphorase histochemical staining was used to detecte the NOS-positive cells.RESULTS:The numbers of NOS-positive cells in cortex and hippocampus increased significantly in ischemia-reperfusion rats,which was inhibited by Rhy(12.5 and 25mg/kg ip,30min befor ischemia).The effect of Rhy was similar to that of L-NAME,a inhibitor of NOS.CONCLUSION:Rhy may protect brain from ischemic injury by inhibition of NOS druing cerebral ischemia-reperfusion.