摘要
目的通过观察同型半胱氨酸（Ｈｃｙ）对血小板Ｌ精氨酸（ＬＡｒｇ）／一氧化氮（ＮＯ）系统的影响，探讨Ｈｃｙ对血小板损伤的机制。方法健康成人６例，平均年龄（３３±７）岁。晨取静脉血，将每例血样分为对照组及加Ｈｃｙ组，分别测定血小板ＬＡｒｇ转运功能；同时将上述两组分别设立加乙酰胆碱（Ａｃｈ）与不加Ａｃｈ组，测定血小板ＮＯ合酶（ＮＯＳ）活性、ＮＯ生成量和ｃＧＭＰ含量。结果（１）在不同浓度的Ｌ－Ａｒｇ时，Ｈｃｙ组ＬＡｒｇ转运速率均低于对照组（Ｐ＜００１）。（２）在Ａｃｈ刺激下，ＮＯＳ活性明显提高，但Ｈｃｙ组提高的程度明显低于对照组（Ｐ＜００１）。（３）在Ａｃｈ刺激下，ＮＯ生成及ｃＧＭＰ含量明显提高（Ｐ＜００１），但Ｈｃｙ组仍明显低于对照组（Ｐ＜００５）。结论Ｈｃｙ影响血小板功能可能与ＬＡｒｇ／ＮＯ系统改变有关。
Objective To probe the mechanism
of damage of platelet function caused by homocysteine (Hcy), by observing the change of the
Larginine/nitric oxide (LArg/NO) pathway of platelet Methods Six normal subjects were
included Plateletrich plasma was prepared Each sample was divided into group H (with Hcy)
and group C (without Hcy) The velocity of LArg transport of platelet was measured Group C
and H were further divided into group C1 (with Ach), C0 (without Ach) and H1 (with Ach) and
H0 (without Ach), respectively The activity of nitric oxide synthase (NOS) and the product of
NO-2 and cGMP were measured in each group Results (1)At different concentrations of LArg,
the velocity of LArg transport in group H was lower than that in group C(P<001) (2)The activity
of NOS, the product of NO-2 and cGMP were increased when stimulated by Ach, but the
level in group H1 was still lower than that in group C1 (P<005) Conclusion The damage of
platelet function caused by Hcy may be related to the change of LArg/NO system in platelet
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
1999年第3期210-212,共3页
Chinese Journal of Cardiology
基金
国家教委科研基金
自然科学基金
