期刊文献+

Identification of novel cyclic nucleotide phosphodiesterase gene cDNAs in the brain of guinea pig

豚鼠脑中表达的磷酸二酯酶基因cDNA序列的鉴定与分析(英文)
原文传递
导出
摘要 Objective Cyclic nucleotide phosphodiesterase(PDE)is a critical component of the nitric oxide(NO)signaling pathway and plays critical roles in cognition and learning,Parkinson’s disease,attention deficit hyperactivity disorder, psychosis and depression.The PDEs in the brain of guinea pig have not yet been reported.The present study aimed to detect the unknown Pde cDNAs in the brain of guinea pig.Methods Reverse transcription polymerase chain reaction(RT-PCR)and sequence comparison analysis were performed to detect the expression of Pde cDNAs and to assess the identity rates of cDNA and amino acid sequences between guinea pig and human or mouse,respectvely.The RT-PCR primers were located on the conserved region of human PDE and mouse Pde cDNAs.Results Eleven novel Pde cDNAs were detected in the brain of guinea pig(Cavia porcellus),including CpPde1a,CpPde1b,CpPde2a,CpPde4a,CpPde4d,CpPde5a,CpPde6c,CpPde7b, CpPde8a,CpPde9a,and CpPde10a.The identity rates of the Pde cDNA sequences between guinea pig and human ranged from 83.8%to 94.3%,and those of the amino acid sequences ranged from 91.9%to 100%.The identity rates of Pde cDNA sequences between guinea pig and mouse ranged from 84.6%to 92.1%,and those of amino acid sequences ranged from 91.2% to 99.2%.The average identity rate of the 11 Pde cDNA sequences between guinea pig and human was significantly higher(P 0.01)than that between guinea pig and mouse.The putative partial amino acid sequences of guinea pig contained at least one of the conserved domains of human and mouse PDE proteins.Conclusion These results indicate that the brainexpressed Pde genes are identified in guinea pig,which lays the foundation for further investigating the physiological roles of PDE proteins in the brain. 目的环核苷酸磷酸二酯酶(phosphodiesterase,PDE)是一氧化氮(nitric oxide,NO)信号转导途径中的一个关键成员,在认知与学习、帕金森病、注意缺陷多动障碍、精神病和抑郁症等过程中发挥重要作用。PDE在豚鼠脑中的表达情况还未见报道。本研究旨在对豚鼠脑中表达的Pde cDNA序列进行鉴定与分析。方法根据人PDE和小鼠Pde cDNA序列的22个保守区设计引物,采用RT-PCR法扩增豚鼠脑中表达的未知Pde基因cDNA部分序列,并采用生物信息学软件分析不同物种间Pde cDNA序列及氨基酸多肽序列的同源性及保守功能基序。结果在豚鼠脑中发现了11个新的Pde基因cDNAs,包括CpPde1a、CpPde1b、CpPde2a、CpPde4a、CpPde4d、CpPde5a、CpPde6c、CpPde7b、CpPde8a、CpPde9a和CpPde10a。豚鼠与人之间的Pde基因cDNA序列的同源率在83.8%-94.3%之间,氨基酸序列的同源率在91.9%-100%之间。豚鼠与小鼠之间的Pde基因cDNA序列的同源率在84.6%-92.1%之间,氨基酸序列的同源率在91.2%-99.2%之间。此外,这11个Pde cDNAs在豚鼠与人之间的平均同源率显著高于在豚鼠与小鼠之间的同源率(P<0.01)。豚鼠的部分氨基酸序列具有至少一个人及小鼠PDE蛋白的保守功能区。结论这些发现为进一步研究PDE在大脑中的生理功能打下了基础。
出处 《Neuroscience Bulletin》 SCIE CAS CSCD 2010年第5期365-370,共6页 神经科学通报(英文版)
基金 supported by the National Natural Science Foundation of China(No.31070928,30600198) the Natural Science Foundation of Guangdong Province,China(No.06301101) the Medical Research Program of Guangdong Province,China(No.A2010259)
关键词 cyclic nucleotide phosphodiesterase the central nervous system nitric oxide guinea pig cross-species comparison 环核苷酸磷酸二酯酶 中枢神经系统 一氧化氮 豚鼠 种间比较
  • 相关文献

参考文献15

  • 1Esplugues JV. NO as a signalling molecule in the nervous system. Br J Pharmacol 2002, 135(5): 1079-1095.
  • 2Essayan DM. Cyclic nucleotide phosphodiesterases. J Allergy Clin Immunol 2001, 108(5): 671-680.
  • 3Lugnier C. Cyclic nucleotide phosphodiesterase (PDE) superfamily:a new target for the development of specific therapeutic agents. Pharmacol Ther 2006, 109(3): 366-398.
  • 4Bender AT, Beavo JA. Cyclic nucleotide phosphodiesterases: molecular regulation to clinical use. Pharmacol Rev 2006, 58 (3): 488-520.
  • 5Kleppisch T. Phosphodiesterases in the central nervous system. Handb Exp Pharmacol 2009, 191: 71-92.
  • 6West KL, Fernandez ML. Guinea pigs as models to study the hypocholesesterolemic effects of drugs. Cardiovasc Drug Rev 2004, 22(1): 55-70.
  • 7Fernandez ML, Volek JS. Guinea pigs: A suitable animal model to study lipoprotein metabolism, atherosclerosis and inflammation. Nutr Metab (Lond) 2006, 3: 17.
  • 8Huang MQ, Zhang JG, Long YS. Identification of novel cyclic nucleotide phosphodiesterase gene cDNAs in the cochlea of guinea pig (Cavia porcellus) through conserved homologous sequences. Mol Biotechnol 2010, 44(1): 66-70.
  • 9Kim LK, Choi UY, Cho HS, Lee JS, Lee WB, Kim J, et al. Down- regulation of NF-kappaB target genes by the AP-1 and STAT complex during the innate immune response in Drosophila. PLoS Biol 2007, 5(9): e238.
  • 10Marchler-Bauer A, Anderson JB, Chitsaz F, Derbyshire MK, DeWeese-Scott C, Fong JH, et al. CDD: specific functional an- notation with the Conserved Domain Database. Nucleic Acids Res 2009, 37: D205-210.

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部