电针对帕金森病大鼠黑质细胞形态及凋亡的影响

Effect of Electroacupuncture on Morphological Changes and Apoptosis of Substantia Nigra Cells in Parkinson's Disease Rats

目的:观察针刺对帕金森病(PD)大鼠黑质多巴胺(DA)能神经元的形态数量及黑质细胞凋亡率的影响,探讨电针治疗PD的部分作用机制。方法:Wistar大鼠40只随机分为正常组、假手术组、模型组和电针组,以6-羟基多巴胺右侧纹状体微量注射法制备PD大鼠旋转模型。电针组取"风府""太冲"电针治疗,每日1次,共治疗2周。采用HE染色观察各组黑质的形态学变化,以Nissl染色检测各组黑质的神经元总数,以免疫组织化学法检测各组黑质、纹状体酪氨酸羟化酶(TH)阳性细胞和纤维的数量,采用流式细胞术检测各组黑质TH阳性细胞百分比及细胞凋亡比例。结果:模型组损毁侧黑质致密部神经元总数、TH阳性神经元数量及纹状体TH阳性神经纤维数量显著低于正常组和假手术组(P<0.01),电针组与模型组比较各相应指标数值均显著增加(P<0.01);电针组损毁侧黑质细胞凋亡率显著低于模型组(P<0.01),但仍较正常组和假手术组显著增高(P<0.01)。结论:电针治疗能显著提高PD模型大鼠黑质DA能神经元数量及纹状体DA能神经纤维密度,显著降低PD模型鼠黑质细胞凋亡率,说明电针干预对PD模型鼠黑质纹状体系统多巴胺能神经元具有明显的保护作用。

Objective To observe the influence of electroacupuncture （EA） of ＂Fengfu＂（GV 16） and ＂Taichong＂（LR 3） on morphological changes and apoptotic percentage of substantia nigra cells so as to explore its underlying mechanism in relieving Parkinson＇s disease. Methods Forty Wistar rats were randomly divided into normal control, sham-operation （sham）, model and EA groups, with 10 rats in each. The Parkinson＇s disease model was established by micro-injection of 6-hydroxyl-dopamine （6-OHDA） into the right striatum. EA （2 Hz, 1 mA） was applied to ＂Fengfu＂（GV 16） and ＂Taichong＇（LR 3） for 30 min, once daily for 2 weeks. HE staining, Nissl staining and immunohistochemical staining were conducted for observing the morphological changes of substantia nigra pars compacta （SNc） and striatum, and the tyrosine hydroxylase（＇TH） immuno-reaction （IR） positive neurons and nerve fibers. The apoptosis was detected by flow cytometry. Results Compared to the normal control and sham groups, the total number of TH-IR positive neurons and Nissl-stained cells, and OD value of TH-IR positive nerve fibers in the SNc on the 6-OHDA-lesioned side were significantly lower in the model group （P〈0.01 ）. Compared with the model group, the aforementioned 3 indexes of the EA group were increased obviously （P〈0.01）. The percentage of apoptosis of SNc on the 6-OHDA- lesioned side was obviously lower in the EA group than in the model group （P〈0.01）, but still obviously higher than in the normal group and sham group （P〈0. 01）. Conclusion Electroacupuncture therapy can significantly increase the number of neurons of substantia nigra and the density of striatum nerve fibers, and reduce the apoptotic percentage of substantia nigra cells in the Parkinsonian rats, which may contribute to its effect in relieving Parkinson＇s disease.