摘要
为探讨人巨细胞病毒（ＨｕｍａｎＣｙｔｏｍｅｇａｌｏｖｉｒｕｓ，ＨＣＭＷ）ＡＤ１６９毒株体外感染兔胚肺细胞（ＲａｂｂｉｔＥｍｂｒｙｏＬｕｎｇｃｅｌ，ＲＥＬ）的敏感性，建立ＨＣＭＶＡＤ１６９毒株感染模型，将ＨＣＭＶＡＤ１６９毒株定量接种于ＲＥＬ，观察细胞病理变化；用单克隆抗体－免疫组化方法检测细胞内ＨＣＭＶ抗原；电镜检测细胞内病毒颗粒，用ＥＬＩＳＡ方法检测经ＲＥＬ增殖病毒培养物ＨＣＭＶ抗原滴度，用人胚肺细胞（ＨｕｍａｎＥｍｂｒｙｏＬｕｎｇｃｅｌ，ＨＥＬ）滴定病毒毒力变化。结果表明，接种病毒后，ＲＥＬ于４８ｈ出现细胞肿胀，胞浆内出现折光性颗粒，逐渐变圆、脱落，其病变特征与该病毒在ＨＥＬ增殖出现的病变一致；用免疫组化法在胞浆及其核内查到ＨＣＭＶ抗原；用透射电镜观察，在细胞核内查到大量病毒颗粒；用ＥＬＩＳＡ方法在ＲＥＬ病毒培养物中查到ＨＣＭＶ抗原，抗原滴度与ＨＥＬ增殖的病毒一致；与ＨＥＬ增殖物比较，经ＲＥＬ增殖的病毒培养物毒力降低１００ＴＣＩＤ５０。表明ＲＥＬ是ＨＣＭＶＡＤ１６９毒株的敏感宿主细胞。
The sensitivity of HCMV AD169 stain infect rabbits embryo lung cells(REL) was studied, and an infection model of HCMV was established. HCMV AD169 was quantilatively inoculated in the rabbit embryo lung cells(REL) monolayer originated from New Zealand rabbit, Belgium hare, Californian rabbit, Japanese rabbit, Haerbin white rabbit, Chinese native rabbit and hybrid rabbit. The cytopathic effect(CPE) appeared in 48h after inoculation, which was similar to that of the human embryo lung cells(HEL) infected by the HCMV AD169 strain. HCMV antigen was detected in the nucleus and plasm by immunohistochemistry and a large number of viral particulates were manifested in the nuclei of the cells that had CPE by electron microscope. The results demonstrated that embryo lung cells from several species of rabbits were susceptible to HCMV AD169 strain and manifested typical CPE after inoculation, which could active as an infection model of HCMV.
出处
《山东医科大学学报》
1999年第2期109-111,共3页
Acta Academiae Medicinae Shandong
基金
山东省自然科学基金
