病毒性肝炎中庚肝病毒感染的分子流行病学调查

INVESTIGATION ON HEPATITIS G VIRUS INFECTION IN PATIENTS WITH VIRAL HEPATITIS

为探讨山东地区各型病毒性肝炎中庚型肝炎病毒（ＨＧＶ）的感染状况，采用逆转录－套式聚合酶链反应法（ＲＴ－ｎＰＣＲ）检测１５９例病毒性肝炎血清ＨＧＶ－ＲＮＡ。结果表明：甲型和戊型肝炎ＨＧＶ－ＲＮＡ均阴性。乙型肝炎ＨＧＶ－ＲＮＡ的检出率，在急性肝炎（０／２０）、慢性肝炎（２／５０）、肝硬变（１／１０）和慢性重型肝炎（１／５）依次升高。慢性丙型肝炎ＨＧＶ－ＲＮＡ的检出率为３０．０％。非甲－戊（ＮＡ－Ｅ）型肝炎ＨＧＶ－ＲＮＡ的检出率为２３．３％，且在急性肝炎（２／１５）、慢性肝炎（２／８）、肝硬变（３／５）中依次升高。肝细胞癌４例，２例ＨＧＶ－ＲＮＡ阳性。本组ＨＧＶ－ＲＮＡ阳性１９例中，８例有输血史，７例有多次住院史，４例感染来源不清。提示ＨＧＶ感染常见于乙、丙、非甲－戊型肝炎和肝癌中，其感染途径可能与输血和医院内传播有关。

In order to investigate the prevalence of hepatitis G virus(HGV) infection in patients with viral hepatitis in Shandong. Sera HGVRNA were detected in 159 patients with different types of hepatitis by a reverse transcription nested polymerase chain reaction (RTnPCR). The results showed that the HGVRNA was negative in patients with hepatitis A and hepatitis E. The positive rates of HGVRNA in patients with acute hepatitis B, chronic hepatitis B, liver cirrhosis caused by HBV, chronic severe hepatitis B were 0(0/20),40%(2/50),100%(1/10) and 20.0%(1/5)respectively. The positive rate of HGVRNA in patients with chronic hepatitis C was 30.0%(6/20). Among patients with non AE hepatitis, the positive rates of HGVRNA in acute hepatitis, chronic hepatitis and liver cirrhosis were 13.3%(2/15),25.0%(2/8) and 60.0%(3/5) respectively; and the total positive rate was 23.3%(7/30). 2 of 4 serum samples from patients with hepatocellular carcinoma were HGVRNA positive. Among 19 patients of HGVRNA positive, 8 patients had a history of blood transfusion and 7 patients had been hospitalized more than three times, the transmitted route in the other 4 patients was unclear. The results indicate that HGV infection is common in hepatitis B, hepatitis C,non AE hepatitis and hepatocellular carcinoma, and obviously, HGV infection is related to blood transfusion and iatragenic transmission.