左氧氟沙星注射剂含量及有关物质的HPLC测定

DETERMINATION OF LEVOFLOXACIN INJECTION AND \=RELATED SUBSTANCES BY HPLC

以反相高效液相色谱法测定左氧氟沙星注射剂的含量及有关物质。采用ＷａｔｅｒｓＮｏｖａ－ＰａｋＣ１８柱，１０ｍｍｏｌ／ＬＫＨ２ＰＯ４－１０ｍｍｏｌ／Ｌ溴化四丁铵－乙腈（４５∶４５∶１０，磷酸调节ｐＨ２．８）为流动相，检测波长为２９３ｎｍ。平均回收率为１００．５％，ＲＳＤ为０．７１％。方便简便、准确。

A reverse phase HPLC method was described for determination of levofloxacin injection and related substances. Waters NovaPak C18 column(3.9150 mm,4 m) and mobile phase, 10 mmol/L KH2PO410 mmol/L(C4H9)4NBrCH3CN(454510,pH2.8), were used. Detection wavelength was 293 nm. The average recovery was 100.5% with RSD of 0.71%.