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重组人促红细胞生成素(rhEPO)对帕金森病大鼠模型小胶质细胞活化的影响 被引量：1

Effects of recombinant human erythropoietin on microglia in rat model of Parkinson's disease
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摘要 目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)对6-羟基多巴胺(6-OHDA)诱导的SD大鼠帕金森病(PD)模型小胶质细胞活化的影响。方法 40只SD大鼠随机分为A组(rhEPO+6-OHDA)、B组(生理盐水+6-OHDA)、C组(6-OHDA)、D组(生理盐水),每组10只。(1)A组:右侧纹状体内立体定向注射重组促红细胞生成素(rhEPO),24h后同侧黒质内立体定向注射6-OHDA;(2)B组:右侧纹状体内立体定向注射与rhEPO等量的生理盐水,24h后同侧黒质内立体定向注射6-OHDA;(3)C组:右侧黒质内立体定向注射6-OHDA;(4)D组:右侧黒质内立体定向注射与6-OHDA等量的生理盐水。4w后采用免疫组化检测黒质内酪氨酸羟化酶(TH)阳性神经元和CD11b阳性细胞数量及CD11b阳性细胞形态变化。结果与D组比较,A组大鼠黒质TH阳性神经元明显减少,CD11b阳性细胞明显增多,大部分小胶质细胞胞体小,突起细长;与B组和C组比较,A组大鼠黒质TH阳性神经元显著增多,CD11b阳性细胞显著减少,仅有少量小胶质细胞胞体大,突起短粗。结论重组人促红细胞生成素(rhEPO)可能通过抑制小胶质细胞活化,减轻6-OHDA对多巴胺(DA)能神经元的毒性损害,对DA能神经元产生神经保护作用。 Objective To study the effects of recombinant human erythropoietin（rhEPO）on microglia in rat model of Parkinson＇s disease induced by 6-hydroxydopamine（6-OHDA）.Methods Forty SD rats were randomly divided into four groups：A group（rhEPO＋6-OHDA）,B group（saline＋6-OHDA）,C group（6-OHDA）and D group（saline）,ten average each group.Related manipulation：A group,ipsilateral substantia nigra（SN）stereotactic rejection of 6-OHDA on 24h after right striatum stereotactic rejection of rhEPO;B group,ipsilateral SN stereotactic rejection of 6-OHDA on 24h after right striatum stereotactic rejection of saline（equal quantity of rhEPO）;C group,right SN stereotactic rejection of 6-OHDA;D group,right SN stereotactic rejection of saline（equal quantity of 6-OHDA）.All groups were detected 4 weeks after the operation about the numbers of tyrosine hydroxylase（TH）positive neurons and morphological changes of CD11b positive cells in the SN by immunohistochemistry.Results Compared with D group,the number of TH＋ neurons of SN reduced greatly in A group,the number of CD11b＋ cells in SN increased markedly and most microglia showed small cell body with slim and long processes in A group.Compared with B and C group,the number of TH＋ neurons in SN increased remarkably in A group,the number of CD11b＋ cells of SN decreased markedly and only a few microglia showed big cell body with thicker shorter processes in A group.Conclusion Probably through blocking activation of microglia,rhEPO could prevent 6-OHDA-induced degeneration of DA neurons and protect DA neurons.
作者 朱红灿 罗常月 张华 赵静 李倩 赵鹏 任秀花 臧卫东
机构地区 郑州大学第一附属医院神经内科
出处 《中风与神经疾病杂志》 CAS CSCD 北大核心 2010年第11期992-994,共3页 Journal of Apoplexy and Nervous Diseases
关键词 重组人促红细胞生成素 帕金森病 小胶质细胞 神经保护 Recombinant human erythropoietin Parkinson＇s disease Microglia Neuroprotection
分类号 R742.5 [医药卫生—神经病学与精神病学]
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参考文献9

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同被引文献39

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中风与神经疾病杂志
2010年 第11期

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