沈阳地区汉族人群脑梗死患者ALOX5AP基因SNP分析

Relationship between ALOX5AP gene single nucleotide polymorphism and stroke of Han population in Shenyang City

目的研究沈阳地区汉族人群5-脂氧合酶激活蛋白基因(ALOX5AP基因)单核苷酸多态(singlenucleotide polymorphism,SNP)与脑梗死易患的相关性。方法收集2008年度沈阳地区常住汉族人群380例脑梗死患者,以及同期随机抽取的425例门诊体检正常对照者,通过多重PCR(Multiplex PCR)获得含有待检测突变位点的基因片断,然后进行多重酶检测反应(Multiplex LDR),最后通过测序仪电泳读取检测结果。并且进行血脂、血糖等的生化指标检查。多元logic回归分析方法校正传统危险因素后分析基因多态性和脑梗死之间的相关独立性。结果共检测7个基因位点的多态变化,在SG13S114,以及SG13S32位点脑梗死组和对照组有显著差异(OR=1.8,P<0.01;OR=1.4,P<0.01)。其它位点SG13S25、SG13S89、G13S32、SG13S35、SG13S41两组之间无差异,单倍体HapA在两组之间也无显著差异。回归分析显示高血压和SG13S114、SG13S32是沈阳地区汉族人群的独立危险因素。结论 ALOX5AP基因的SG13S114、SG13S32和脑梗死相关,是脑梗死的独立危险因素之一。

Objective To study Shenyang Han population 5-lipoxygenase activating protein（ALOX5AP） gene single nucleotide polymorphism（SNP）and susceptibility to stroke.Methods 380 cases of patients with stroke residented in shenyang city in 2008 were collected,as well as 425 cases of age-matched normal control in the same period.The gene fragment contained mutation was detected through multiple PCR（Multiplex PCR）,and then multi-enzyme reaction（Multiplex LDR）was performed.The final test results were read through the sequencer electrophoresis.And lipids,glucose and other biochemical indexes were detected.Multiple logic regression analysis after correction of traditional risk factors analysis was performed to detect the relationship between gene polymorphism and stroke.Results Seven loci polymorphic changes were detected,which in the SG13S114 and SG13S32 sites showed significant difference between stroke and control（OR=1.8,P〈0.05;OR=1.4,P〈0.05）.Other sites,such as SG13S25,SG13S89,G13S32,SG13S35 and SG13S41 showed no difference between the two groups and haploid HapA between the two groups had no significant difference.Regression analysis showed that high blood pressure,and the SNP loci SG13S114 and SG13S32 are independent risk factors in Shenyang Han crowd.Conclusion ALOX5AP gene SNP loci SG13S114 and SG13S32 is related to stroke,and is an independent risk factor for stroke.